Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,254,550	T→C	intergenic (+25/+30)	ychH → / ← dauA	stress‑induced protein YchH/C4‑dicarboxylic acid transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,254,550	0	T	C	100.0%	23.7 / NA	8	intergenic (+25/+30)	ychH/dauA	stress‑induced protein YchH/C4‑dicarboxylic acid transporter DauA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/4);  total (4/4)

GTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGT  >  NZ_CP009273/1254472‑1254628                                                                               |                                                                               ggtCGCCACTATGCCAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGCCAGTTTGCGCt                                                                     >  2:498313/3‑90 (MQ=255)    cGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGCCAGTTTGCGCTGAc                                                                  <  1:335686/90‑1 (MQ=255)    cGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGCCAGTTTGCGCTGAc                                                                  <  2:275525/90‑1 (MQ=255)                 aaCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGCCAGTTTGCGCTGACTGGTCAATCTCGt                                                     <  1:117427/90‑1 (MQ=255)                     ttGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGCCAGTTTGCGCTGACTGGTCAATCTCGTACtt                                                 >  2:180794/1‑90 (MQ=255)                                                                    ttGCTGACCAGCCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGc    >  1:151952/1‑88 (MQ=255)                                                                    ttGCTGACCAGCCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGc    <  2:151952/88‑1 (MQ=255)                                                                    ttGCTGACCAGCCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGt  >  2:80059/1‑90 (MQ=255)                                                                               |                                                                               GTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGT  >  NZ_CP009273/1254472‑1254628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑