Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,279,258	A→G	I67V (ATC→GTC)	narH →	nitrate reductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,279,258	0	A	G	100.0%	23.6 / NA	8	I67V (ATC→GTC)	narH	nitrate reductase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/3);  total (5/3)

GGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCTAACCCGCATCTG  >  NZ_CP009273/1279178‑1279341                                                                                 |                                                                                    ggTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGGTCCGTaaaa                                                                            >  1:395943/1‑90 (MQ=255)      aacaacGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGGTCCGTAAAATCAAc                                                                       <  2:71748/90‑1 (MQ=255)                       gCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGGTCCGTAAAATCAACGGCAAACTGCAGCcgcg                                                      <  2:238377/90‑1 (MQ=255)                           ggCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGGTCCGTAAAATCAACGGCAAACTGCAGCCGCGCa                                                    >  1:328526/1‑88 (MQ=255)                           ggCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGGTCCGTAAAATCAACGGCAAACTGCAGCCGCGCa                                                    <  2:328526/88‑1 (MQ=255)                                             gactgGGAAAACCAGGAAAAATACAAAGGCGGCTGGGTCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCAtgctg                                >  1:56892/1‑90 (MQ=255)                                                                         gcggcTGGGTCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCTAACCCGCATc    >  1:403956/1‑90 (MQ=255)                                                                           ggcTGGGTCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCTAACCCGCATCTg  >  1:80939/1‑90 (MQ=255)                                                                                 |                                                                                    GGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAACCGTGCCATGCTGCTGGGTAAAATCTTCGCTAACCCGCATCTG  >  NZ_CP009273/1279178‑1279341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑