Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,293,696	A→C	intergenic (‑119/‑274)	adhE ← / → ychE	bifunctional acetaldehyde‑CoA/alcohol dehydrogenase/protein YchT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,293,696	0	A	C	100.0%	28.7 / NA	9	intergenic (‑119/‑274)	adhE/ychE	bifunctional acetaldehyde‑CoA/alcohol dehydrogenase/protein YchT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/3);  total (6/3)

TAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTC  >  NZ_CP009273/1293637‑1293781                                                            |                                                                                      taGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTAAAAGAGTTTAAAc                                                         >  2:391465/1‑90 (MQ=255)       gTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTAAAAGAGTTTAAACATTaga                                                   >  2:14312/1‑90 (MQ=255)       gTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTAAAAGAGTTTAAACATTaga                                                   >  2:193617/1‑90 (MQ=255)       gTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTAAAAGAGTTTAAACATTaga                                                   >  2:76596/1‑90 (MQ=255)                   tttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTaaaa                                                                   >  1:65061/1‑62 (MQ=255)                   tttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTaaaa                                                                   <  2:65061/62‑1 (MQ=255)                           aCCTATGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTc                               <  1:391465/90‑1 (MQ=255)                                tGAAAATCAAAAGCTTACTCGCGCTCACCCTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTc                          >  1:218453/1‑90 (MQ=255)                                                        tCACCCTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTc  <  2:238757/90‑1 (MQ=255)                                                            |                                                                                      TAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTC  >  NZ_CP009273/1293637‑1293781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑