Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,295,255	A→C	intergenic (‑100/‑184)	BW25113_RS25865 ← / → oppA	hypothetical protein/oligopeptide ABC transporter substrate‑binding protein OppA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,295,255	0	A	C	100.0%	24.0 / NA	8	intergenic (‑100/‑184)	BW25113_RS25865/oppA	hypothetical protein/oligopeptide ABC transporter substrate‑binding protein OppA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (4/4);  total (4/4)

ATTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCGACAGGGGAGACACAGTACGAATCGACATAAGGTGATCGTCTGAATCACCAGAATAAATAAAGTCGGTGATAGT  >  NZ_CP009273/1295184‑1295320                                                                        |                                                                  aTTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCGACAGGGGCGACACAGTACGAATCGAc                                                 >  2:54231/1‑90 (MQ=255)                     ataatTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCGACAGGGGCGACACAGTACGAATCGACATAAGGTGATCGTCTGAATc                             >  1:452967/1‑90 (MQ=255)                                   aaGAGCGTCGCGAAAGAATAATGTGTCTCGACAGGGGCGACACAGTACGAATCGACATAAGGTGATCGTCTGAATCACCAGAataaataa               <  1:65644/90‑1 (MQ=255)                                       gCGTCGCGAAAGAATAATGTGTCTCGACAGGGGCGACACAGTACGAATCGACATAAGGTGATCGTCTGAATCAcc                          >  1:392844/1‑75 (MQ=255)                                       gCGTCGCGAAAGAATAATGTGTCTCGACAGGGGCGACACAGTACGAATCGACATAAGGTGATCGTCTGAATCAcc                          <  2:392844/75‑1 (MQ=255)                                                aaGAATAATGTGTCTCGACAGGGGCGACACAGTACGAATCGACATAAGGTGATCGTCTGAATCACCAGAATAAATAAAGTCGGTGATAGt  <  1:424765/90‑1 (MQ=255)                                                              tcGACAGGGGCGACACAGTACGAATCGACATAAGGTGAt                                       <  1:203934/39‑1 (MQ=38)                                                              tcGACAGGGGCGACACAGTACGAATCGACATAAGGTGAt                                       >  2:203934/1‑39 (MQ=38)                                                                        |                                                                  ATTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCGACAGGGGAGACACAGTACGAATCGACATAAGGTGATCGTCTGAATCACCAGAATAAATAAAGTCGGTGATAGT  >  NZ_CP009273/1295184‑1295320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑