Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,296,392	A→G	A318A (GCA→GCG)	oppA →	oligopeptide ABC transporter substrate‑binding protein OppA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,296,392	0	A	G	100.0%	67.1 / NA	20	A318A (GCA→GCG)	oppA	oligopeptide ABC transporter substrate‑binding protein OppA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

CTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAC  >  NZ_CP009273/1296324‑1296474                                                                     |                                                                                   cTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACcgc                                                               <  1:273053/90‑1 (MQ=255)  tgtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACcgcg                                                              >  2:10476/1‑90 (MQ=255)   gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACt                                                                            <  1:130122/75‑1 (MQ=255)   gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACt                                                                            >  2:130122/1‑75 (MQ=255)   gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGa                                                             >  2:10028/1‑90 (MQ=255)   gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGa                                                             >  1:136846/1‑90 (MQ=255)   gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGa                                                             >  2:344638/1‑90 (MQ=255)   gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGa                                                             >  2:434219/1‑90 (MQ=255)          tACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCAtt                                                      <  1:344638/90‑1 (MQ=255)          tACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCAtt                                                      >  2:2701/1‑90 (MQ=255)                      aTTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTg                                          >  2:521927/1‑90 (MQ=255)                                       gCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACAt                         >  2:27918/1‑90 (MQ=255)                                       gCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACAt                         >  2:40839/1‑90 (MQ=255)                                          aTTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGGGAAAGCGCAGGGCAACATGcc                      <  1:434219/90‑1 (MQ=255)                                                 gATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAt               <  1:10028/90‑1 (MQ=255)                                                    gtgcgtgtgcgtACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGt            >  2:398052/1‑90 (MQ=255)                                                         tgtgcgtACCGCGCTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTacac       <  2:130201/90‑1 (MQ=255)                                                           tgcgtACCGCGCTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTc     <  1:398052/90‑1 (MQ=255)                                                              gtACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAc  <  1:27918/90‑1 (MQ=255)                                                              gtACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAc  <  1:40839/90‑1 (MQ=255)                                                                     |                                                                                   CTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAC  >  NZ_CP009273/1296324‑1296474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑