Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	159,790	A→G	E400G (GAG→GGG)	hrpB →	ATP‑dependent helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	159,790	0	A	G	100.0%	27.7 / NA	9	E400G (GAG→GGG)	hrpB	ATP‑dependent helicase HrpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/7);  total (2/7)

CAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCG  >  NZ_CP009273/159711‑159870                                                                                |                                                                                 cAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACgg                                                                        <  2:128090/90‑1 (MQ=255)        gctggctgGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTg                                                                 >  2:427592/1‑90 (MQ=255)                                tGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcgc                                         <  1:379032/90‑1 (MQ=255)                                tGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcgc                                         <  1:427592/90‑1 (MQ=255)                                tGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcgc                                         <  2:513090/90‑1 (MQ=255)                                       aCTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTaa                                  <  1:178208/90‑1 (MQ=255)                                                    cGTCTGTTACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTa                     <  2:486484/90‑1 (MQ=255)                                                           tACAAATGCTGGGGGCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCaa              <  2:154301/90‑1 (MQ=255)                                                                       gggCACTGGGGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAgcg  >  2:119352/1‑90 (MQ=255)                                                                                |                                                                                 CAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCG  >  NZ_CP009273/159711‑159870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑