Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,333,464	A→G	intergenic (‑47/‑123)	ribA ← / → pgpB	GTP cyclohydrolase II/phosphatidylglycerophosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,333,464	0	A	G	100.0%	18.9 / NA	7	intergenic (‑47/‑123)	ribA/pgpB	GTP cyclohydrolase II/phosphatidylglycerophosphatase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/3);  total (4/3)

TTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTT  >  NZ_CP009273/1333381‑1333548                                                                                    |                                                                                     ttGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATGACgaga                                                                                <  1:31896/90‑1 (MQ=255)                                  gCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATGACGAGAAGGGGTTCACCTAATCACGACGCGTCGAt                                                   >  1:512702/1‑86 (MQ=255)                                  gCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATGACGAGAAGGGGTTCACCTAATCACGACGCGTCGAt                                                   <  2:512702/86‑1 (MQ=255)                                  gCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATGACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGtt                                               >  2:170014/1‑90 (MQ=255)                                  gCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATGACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGtt                                               >  2:233883/1‑90 (MQ=255)                                                                    tGGCTTATTTTGCCATGACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTgg             >  2:415700/1‑90 (MQ=255)                                                                               gCCATGACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGttt  <  2:143077/90‑1 (MQ=255)                                                                                    |                                                                                     TTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTT  >  NZ_CP009273/1333381‑1333548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑