Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,339,195	G→A	A602V (GCG→GTG)	pdeR ←	cyclic di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,339,195	0	G	A	100.0%	36.2 / NA	11	A602V (GCG→GTG)	pdeR	cyclic di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

TTCTTGGTTAAAAAAGCATCTTCCTTTGCACTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCGCCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCGAACAAAAACCTGGTCAAGTTTGATGGCATCGATC  >  NZ_CP009273/1339108‑1339272                                                                                        |                                                                              ttCTTGGTTAAAAAAGCATCTTCCTTTGCACTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCAcc                                                                             <  1:317505/90‑1 (MQ=255)          aaaaaaGCATCTTCCTTTGCACTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGt                                                                    >  2:259762/1‑90 (MQ=255)           aaaaaGCATCTTCCTTTGCACTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTg                                                                   <  1:259762/90‑1 (MQ=255)                      tCCTTTGCACTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACa                                                        <  1:226818/90‑1 (MQ=255)                              aCTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACAGGttgttt                                                <  1:12464/90‑1 (MQ=255)                              aCTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACAGGttgttt                                                <  1:205974/90‑1 (MQ=255)                                         cTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACAGGttgttt                                                >  1:224909/1‑79 (MQ=255)                                         cTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACAGGttgttt                                                <  2:224909/79‑1 (MQ=255)                                           tCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCg                                    <  1:162730/89‑1 (MQ=255)                                           tCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCACCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCg                                    >  2:162730/1‑89 (MQ=255)                                                                            acaGCGACGATCACCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCGAACAAAAACCTGGTCAAGTTTGATGGCatcgatc  >  2:96082/1‑90 (MQ=255)                                                                                        |                                                                              TTCTTGGTTAAAAAAGCATCTTCCTTTGCACTCTCTACACCTTCGGCGATCACCTGAAGATTCAATGCCTGGGCCACAGCGACGATCGCCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCGAACAAAAACCTGGTCAAGTTTGATGGCATCGATC  >  NZ_CP009273/1339108‑1339272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑