Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,391,832	C→T	L71L (CTG→TTG)	ynaJ →	DUF2534 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,391,832	0	C	T	100.0%	37.7 / NA	11	L71L (CTG→TTG)	ynaJ	DUF2534 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

TTCCGCTGTCTGAGTGGACGACATCAATGTATGTGATTCAGTCATCGATGATTTTTGTTTATAGCCTGGTCTTTACTGTGTTGCTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTT  >  NZ_CP009273/1391749‑1391916                                                                                    |                                                                                     ttCCGCTGTCTGAGTGGACGACATCAATGTATGTGATTCAGTCATCGATGATTTTTGTTTATAGCCTGGTCTTTACTGTGTTGTTGGCaa                                                                                <  2:207277/90‑1 (MQ=255)                                  tgATTCAGTCATCGATGATTTTTGTTTATAGCCTGGTCTTTACTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAag                                               >  2:16127/1‑90 (MQ=255)                                    aTTCAGTCATCGATGATTTTTGTTTATAGCCTGGTCTTTACTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAagag                                             >  2:66717/1‑90 (MQ=255)                                     ttCAGTCATCGATGATTTTTGTTTATAGCCTGGTCTTTACTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGc                                            >  1:295054/1‑90 (MQ=255)                                         gTCATCGATGATTTTTGTTTATAGCCTGGTCCTTCCTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCagta                                        <  2:319711/90‑1 (MQ=255)                                                     ttttGTTTATAGCCTGGTCTTTACTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAg                            <  2:448517/90‑1 (MQ=255)                                                             ataGCCTGGTCTTTACTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATa                    >  1:78422/1‑90 (MQ=255)                                                                   tGGTCTTTACTGTGTTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCgg              >  2:42349/1‑90 (MQ=255)                                                                              gtgtTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGt   >  1:127990/1‑90 (MQ=255)                                                                              gtgtTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGt   >  1:308189/1‑90 (MQ=255)                                                                              gtgtTGTTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGt   >  2:261329/1‑90 (MQ=255)                                                                               tgttgtTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGtt  >  1:87504/1‑90 (MQ=255)                                                                                    |                                                                                     TTCCGCTGTCTGAGTGGACGACATCAATGTATGTGATTCAGTCATCGATGATTTTTGTTTATAGCCTGGTCTTTACTGTGTTGCTGGCAATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTT  >  NZ_CP009273/1391749‑1391916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑