| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,480,218 | T→C | D967D (GAT→GAC) | hrpA → | ATP‑dependent RNA helicase HrpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,480,218 | 0 | T | C | 100.0% | 54.4 / NA | 16 | D967D (GAT→GAC) | hrpA | ATP‑dependent RNA helicase HrpA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/7); total (9/7) | |||||||||||
CTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGC > NZ_CP009273/1480135‑1480301 | cTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTg < 1:41879/90‑1 (MQ=255) cggcggATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGAt < 1:88726/90‑1 (MQ=255) gATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACaa > 1:259442/1‑90 (MQ=255) tACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCt < 2:65886/90‑1 (MQ=255) tGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAaaga > 2:110430/1‑90 (MQ=255) tGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAaaga > 2:369351/1‑90 (MQ=255) tGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAaaga > 2:453552/1‑90 (MQ=255) gcgAAGACTGGCACTGGGATCAGGTGCCCGaccacc > 1:162457/1‑36 (MQ=37) gcgAAGACTGGCACTGGGATCAGGTGCCCGaccacc < 2:162457/36‑1 (MQ=37) gcgAAGACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGgc > 1:421418/1‑90 (MQ=255) gACTGGCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGcgctcg < 2:188859/90‑1 (MQ=255) gCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACa < 1:366297/90‑1 (MQ=255) gCACTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACa < 2:505059/90‑1 (MQ=255) aCTGGGATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAg < 1:128789/90‑1 (MQ=255) gggATCAGGTGCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATc > 1:26967/1‑90 (MQ=255) gCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGGAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATgc > 1:326710/1‑90 (MQ=255) gCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATgc > 1:68041/1‑90 (MQ=255) gCCCGACCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATgc > 1:98406/1‑90 (MQ=255) | CTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGC > NZ_CP009273/1480135‑1480301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |