Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,506,265	A→G	K119E (AAA→GAA)	ydcS →	ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,506,265	0	A	G	100.0%	47.0 / NA	14	K119E (AAA→GAA)	ydcS	ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/7);  total (7/7)

GCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATTCCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGG  >  NZ_CP009273/1506194‑1506345                                                                        |                                                                                 gCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCcgcgcg                                                                <  2:74884/90‑1 (MQ=255)                        aTGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAAt                                         >  1:346456/1‑90 (MQ=255)                               aaCGCGTGCAGCCGATTAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTggcg                                  >  1:485246/1‑90 (MQ=255)                                            gATTAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACgg                     >  1:4260/1‑90 (MQ=255)                                               tAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGcac                  <  1:495838/90‑1 (MQ=255)                                               tAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGcac                  <  1:84424/90‑1 (MQ=255)                                               tAATACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGcac                  <  2:432044/90‑1 (MQ=255)                                                  tACCGCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACAcc               <  2:376394/90‑1 (MQ=255)                                                      gCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTAc           >  1:171879/1‑90 (MQ=255)                                                      gCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTAc           >  2:342989/1‑90 (MQ=255)                                                      gCATTGATTCCCAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTAc           <  2:346456/90‑1 (MQ=255)                                                               cccAACTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATggggg  >  2:519921/1‑90 (MQ=255)                                                                  aaCTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCAACt                                                 >  1:318379/1‑40 (MQ=38)                                                                  aaCTGGGAAACGCTCGATCCGCGCGTGGTTAAAGGCAACt                                                 <  2:318379/40‑1 (MQ=38)                                                                        |                                                                                 GCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATTCCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGG  >  NZ_CP009273/1506194‑1506345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑