Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,528,286	G→A	T2T (ACG→ACA)	nhoA →	N‑hydroxyarylamine O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,528,286	0	G	A	100.0%	30.2 / NA	10	T2T (ACG→ACA)	nhoA	N‑hydroxyarylamine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

GCGATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACGCCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGCTGCTGCGGTCAATATTGATACGCTTCGTGC  >  NZ_CP009273/1528207‑1528360                                                                                |                                                                           gcgATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGa                                                                  <  1:330422/90‑1 (MQ=255)     tCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGAATCa                                                              <  2:452666/90‑1 (MQ=255)                    ggTGCGGCGTCTATTCCAGGTTATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTAt                                               >  2:80678/1‑90 (MQ=255)                     gTGCGGCGTCTATTCCAGGTTATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTAtt                                              >  1:196792/1‑90 (MQ=255)                                        ttATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGctgctg                           <  1:309917/90‑1 (MQ=255)                                           taAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGCTGCTGCgg                        <  2:404281/90‑1 (MQ=255)                                                tGAGAAAACCACTAAGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGCTGCTGCGGTCAat                   >  1:470653/1‑90 (MQ=255)                                                        ccACTAAGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGCTGCTGCGGTCAATATTGATAc           >  2:312815/1‑90 (MQ=255)                                                              aGGGAAACGCCTGATGACACCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGCTGCTGCGGTCAATATTGATACGCTTCg     <  1:244266/90‑1 (MQ=255)                                                                 gAAACGCCTGATGACACCCATTCTGAATCACTATTCTTCCCGTATTAACTGGTCGGGAGCTGCTGCGGTCAATATTGATACGCTTCGTGc  >  1:154462/1‑90 (MQ=255)                                                                                |                                                                           GCGATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATAAGTTGAGAAAACCACTAAGGGAAACGCCTGATGACGCCCATTCTGAATCACTATTTTGCCCGTATTAACTGGTCGGGAGCTGCTGCGGTCAATATTGATACGCTTCGTGC  >  NZ_CP009273/1528207‑1528360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑