Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,535,292	T→C	E519G (GAA→GGA)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,535,292	0	T	C	100.0%	68.4 / NA	21	E519G (GAA→GGA)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

TACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATC  >  NZ_CP009273/1535205‑1535365                                                                                        |                                                                          tACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGGCCATGCGTTTTATGGGCGGCATCGGCAAATTCACGGGCGATGGTTCCa                                                                         >  2:246362/1‑90 (MQ=25)     aGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAatat                                                                     >  1:35716/1‑90 (MQ=255)     aGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAatat                                                                     >  1:443041/1‑90 (MQ=255)       tGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATAc                                                                   <  2:443041/90‑1 (MQ=255)          ttAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTgg                                                                >  2:117118/1‑90 (MQ=255)          ttAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTgg                                                                >  2:100687/1‑90 (MQ=255)                ccTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATAGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGc                                                          >  1:331057/1‑90 (MQ=255)                  tGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCAc                                                        >  2:71887/1‑90 (MQ=255)                  tGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCAc                                                        <  1:71887/90‑1 (MQ=255)                  tGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCAc                                                        >  1:299461/1‑90 (MQ=255)                   gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCaa                                                                        <  1:263236/73‑1 (MQ=255)                   gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGGTCCaa                                                                        >  2:263236/1‑73 (MQ=255)                           gATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAAt                                               >  2:416972/1‑90 (MQ=255)                                 catcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTc                                         <  2:517751/90‑1 (MQ=255)                                     gAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCAccc                                     <  2:278289/90‑1 (MQ=255)                                          cccATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGc                                <  2:114590/90‑1 (MQ=255)                                                   tttATGGGCGGTATCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTa                       <  2:213218/90‑1 (MQ=255)                                                               aTCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc           <  2:351078/90‑1 (MQ=255)                                                               aTCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc           <  1:117118/90‑1 (MQ=255)                                                               aTCGGCAAATTCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc           <  1:100687/90‑1 (MQ=255)                                                                        ttCACGGGCGATGGTTCCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATc  <  2:187502/90‑1 (MQ=255)                                                                                        |                                                                          TACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATC  >  NZ_CP009273/1535205‑1535365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑