Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,551,234	T→C	I118T (ATC→ACC)	osmC →	peroxiredoxin OsmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,551,234	0	T	C	100.0%	20.3 / NA	7	I118T (ATC→ACC)	osmC	peroxiredoxin OsmC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/2);  total (5/2)

TTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCA  >  NZ_CP009273/1551159‑1551317                                                                            |                                                                                    ttGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAACCCAGaaagcaaaag                                                                       >  1:189827/1‑90 (MQ=255)               aTCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAACCCAGAAAGCAAAAGCAGGATGCCCGGtc                                                         <  1:438921/90‑1 (MQ=255)                    aCTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAACCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCa                                                    <  1:103766/90‑1 (MQ=255)                                                        ctctACCTTTGACGGCATAACCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                >  1:32648/1‑90 (MQ=255)                                                        ctctACCTTTGACGGCATAACCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                >  2:115208/1‑90 (MQ=255)                                                                      gCATAACCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCa  >  1:467361/1‑90 (MQ=255)                                                                      gCATAACCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCa  >  2:327451/1‑90 (MQ=255)                                                                            |                                                                                    TTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCA  >  NZ_CP009273/1551159‑1551317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑