Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,612,612	A→C	V185G (GTG→GGG)	marC ←	NAAT family transporter MarC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,612,612	0	A	C	100.0%	43.0 / NA	13	V185G (GTG→GGG)	marC	NAAT family transporter MarC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/3);  total (10/3)

GCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCA  >  NZ_CP009273/1612547‑1612696                                                                  |                                                                                     gCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGc                                                              >  2:331795/1‑90 (MQ=255)        catacataCCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAg                                                       <  1:187700/90‑1 (MQ=255)              tacCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTcc                                                 >  1:156828/1‑90 (MQ=255)              tacCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTcc                                                 >  1:338462/1‑90 (MQ=255)              tacCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTcc                                                 >  2:122276/1‑90 (MQ=255)                ccAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTcccc                                               >  1:482920/1‑90 (MQ=255)                ccAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTcccc                                               >  2:194557/1‑90 (MQ=255)                                         gaTCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGaaaaa                      >  2:107795/1‑90 (MQ=255)                                         gaTCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGaaaaa                      >  2:153907/1‑90 (MQ=255)                                         gaTCGCTTCAATGCCACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGaaaaa                      >  2:221233/1‑90 (MQ=255)                                                        ccccTTCCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGgcgc       <  2:106518/89‑1 (MQ=255)                                                          ccTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCaa     <  2:482920/90‑1 (MQ=255)                                                             tGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCa  >  1:15455/1‑90 (MQ=255)                                                                  |                                                                                     GCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCA  >  NZ_CP009273/1612547‑1612696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑