| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,651,047 | A→G | V223A (GTC→GCC) | ynfC ← | YnfC family lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,651,047 | 0 | A | G | 95.0% | 61.8 / ‑4.1 | 20 | V223A (GTC→GCC) | ynfC | YnfC family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (11/8); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTA > NZ_CP009273/1650979‑1651131 | tcCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGGCGGCGGGTTTTACTCCTtcat > 1:116751/1‑90 (MQ=255) ccTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTtcatc > 2:397828/1‑90 (MQ=255) cGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGa > 2:480292/1‑90 (MQ=255) cGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGGCGGCGAGTTTTACTCCTTCATCAACAATGa > 2:180140/1‑90 (MQ=255) ttAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGGCGGCGGGtttt > 1:37705/1‑55 (MQ=255) ttAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGGCGGCGGGtttt < 2:37705/55‑1 (MQ=255) ttAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGGCGGCGAGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCAc < 1:180140/90‑1 (MQ=255) gatcgTATTTTTGATGGTGTAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTg > 2:404776/1‑90 (MQ=255) tcgTATTTTTGATGGTGTAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGAc > 1:207414/1‑90 (MQ=255) tGGTGTAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac < 1:480292/90‑1 (MQ=255) gtgtAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGAc < 2:112927/76‑1 (MQ=255) gtgtAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGAc > 1:112927/1‑76 (MQ=255) tAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGctc < 2:116751/90‑1 (MQ=255) tAAACCCGTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGctc < 1:442664/90‑1 (MQ=255) gTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac > 1:8014/1‑78 (MQ=255) gTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac < 1:75394/78‑1 (MQ=255) gTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac > 2:75394/1‑78 (MQ=255) gTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac < 2:8014/78‑1 (MQ=255) gTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTa > 2:182064/1‑90 (MQ=255) gTTCGGCGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTa > 1:68479/1‑90 (MQ=255) | TCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTA > NZ_CP009273/1650979‑1651131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |