Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,657,277	T→C	S11P (TCC→CCC)	dmsB →	dimethylsulfoxide reductase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,657,277	0	T	C	100.0%	24.3 / NA	8	S11P (TCC→CCC)	dmsB	dimethylsulfoxide reductase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/3);  total (5/3)

ACCCGTCGCACAGCAATCTTGTCCAGATCGAAAAGGTTTAAGGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATTCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCAAAGATTTTAAAGATCTTGGCCCG  >  NZ_CP009273/1657196‑1657342                                                                                  |                                                                  aCCCGTCGCACAGCAATCTTGTCCAGATCGAAAAGGTTTAAGGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATCCCAGCCGc                                                           <  1:338613/90‑1 (MQ=255)         cacaGCAATCTTGTCCAGATCGAAAAGGTTTAAGGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATCCCAGCCGCTGTACTgg                                                   <  2:228505/90‑1 (MQ=255)                                    gTTTAAGGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATCCCAGCCGCTGTACTGGCTGTAAAACCTGTGAACTGGCGTGCaa                        >  2:56092/1‑90 (MQ=255)                                     tttAAGGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATCCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCaaa                       <  2:296648/90‑1 (MQ=255)                                             gTAGCCCATGACCACACAATATGGATTTTTTCTCGATCCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCAAAGATTTTaa               >  1:397550/1‑90 (MQ=255)                                                    aTGACCACACAATATGGATTTTTTATCGATCCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCAAAGATTTTAAAGATCtt        >  2:91360/1‑90 (MQ=255)                                                          acacaATATGGATTTTTTATCGATCCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCAAAGATTTTAAAGATCTTGGCCCg  >  1:104848/1‑90 (MQ=255)                                                          acacaATATGGATTTTTTATCGATCCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCAAAGATTTTAAAGATCTTGGCCCg  >  2:44510/1‑90 (MQ=255)                                                                                  |                                                                  ACCCGTCGCACAGCAATCTTGTCCAGATCGAAAAGGTTTAAGGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATTCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGTGCAAAGATTTTAAAGATCTTGGCCCG  >  NZ_CP009273/1657196‑1657342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑