Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,657,602	A→G	E119G (GAA→GGA)	dmsB →	dimethylsulfoxide reductase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,657,602	0	A	G	100.0%	35.7 / NA	11	E119G (GAA→GGA)	dmsB	dimethylsulfoxide reductase subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/4);  total (7/4)

GTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGC  >  NZ_CP009273/1657538‑1657685                                                                 |                                                                                    gTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGAt                                                            <  2:254412/90‑1 (MQ=255)           gCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTAtt                                                  >  2:419930/1‑90 (MQ=255)                        ccACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGa                                     >  1:337654/1‑90 (MQ=255)                         cacaTGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAg                                    >  2:451924/1‑90 (MQ=255)                          acaTGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAgg                                   <  1:14932/90‑1 (MQ=255)                                cTTGTCCGTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAaac                             <  1:159060/90‑1 (MQ=255)                                       gTACGGCGCGCCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCat                      >  1:128138/1‑90 (MQ=255)                                             cgcgcCACAGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATgtgt                >  2:385318/1‑90 (MQ=255)                                                   acaGTACAATGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATc          <  2:337654/90‑1 (MQ=255)                                                           aTGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGc  >  1:19101/1‑90 (MQ=255)                                                           aTGCTGGAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGc  >  1:30532/1‑90 (MQ=255)                                                                 |                                                                                    GTTTGTATCGGCTGCCGCTACTGCCACATGGCTTGTCCGTACGGCGCGCCACAGTACAATGCTGAAAAAGGGCACATGACGAAGTGCGATGGTTGTTATTCGCGCGTCGCCGAGGGGAAACAACCCATATGTGTCGAATCCTGCCCGC  >  NZ_CP009273/1657538‑1657685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑