Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,688,059	G→A	A154A (GCC→GCT)	uidB ←	glucuronide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,688,059	0	G	A	100.0%	36.1 / NA	12	A154A (GCC→GCT)	uidB	glucuronide transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

ACCGACACCATCTCTTCCGGGCTGGAGTTCTTAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCGGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTAGCAAGTGAACCATAAGGAATA  >  NZ_CP009273/1687979‑1688140                                                                                 |                                                                                  aCCGACACCATCTCTTCCGGGCTGGAGTTCTTAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCa                                                                          >  2:103238/1‑90 (MQ=255)   cGACACCATCTCTTCCGGGCTGGAGTTCTTAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCACg                                                                        >  2:212830/1‑90 (MQ=255)    gACACCATCTCTTCCGGGCTGGAGTTCTTAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCACGa                                                                       >  1:476617/1‑90 (MQ=255)       acCATCTCTTCCGGGCTGGAGTTCTTAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCACGAGcc                                                                    <  1:212830/90‑1 (MQ=255)                               ttAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATtgt                                            <  1:432767/90‑1 (MQ=255)                                             tATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCAt                              <  2:197474/90‑1 (MQ=255)                                                   aaaTGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGt                        >  1:134650/1‑90 (MQ=255)                                                    aaTGCCAGGCAGACAAAGGTCAATGAAGCAGCAATCCCCCGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTa                       <  2:475353/90‑1 (MQ=255)                                                                aCAAAGGTCAATGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTAGCAAGTGAACCa           >  1:438235/1‑90 (MQ=255)                                                                     ggTCAATGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTAGCAAGTGAACCATAAgg      <  1:103238/90‑1 (MQ=255)                                                                         aaTGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTAGCAAGTGAACCATAAGGAata  >  1:64732/1‑90 (MQ=255)                                                                         aaTGAAGCAGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTAGCAAGTGAACCATAAGGAata  <  2:14441/90‑1 (MQ=255)                                                                                 |                                                                                  ACCGACACCATCTCTTCCGGGCTGGAGTTCTTAATGCTCGGTCCTATCAGAAATGCCAGGCAGACAAAGGTCAATGAAGCGGCAATCCCACGAGCCGCGCCCAGACGGGCGCGGGATTGTGGTTGTTGGGTCATCGCGGTAGCAAGTGAACCATAAGGAATA  >  NZ_CP009273/1687979‑1688140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑