Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,699,521	G→A	T5T (ACG→ACA)	ydgK →	DUF2569 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,699,521	0	G	A	100.0%	30.8 / NA	11	T5T (ACG→ACA)	ydgK	DUF2569 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

AAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCGTTGTTG  >  NZ_CP009273/1699466‑1699602                                                        |                                                                                  aaaTTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGt                                                 <  1:34644/90‑1 (MQ=255)     tAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGc                                             >  1:475042/1‑90 (MQ=255)                 tttGCGTCGCTATTCTGGAGAACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGt                                 <  1:353564/90‑1 (MQ=255)                      gTCGCTATTCTGGAGAACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGctc                            <  1:227207/90‑1 (MQ=255)                      gTCGCTATTCTGGAGAACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGctc                            <  2:203405/90‑1 (MQ=255)                      gTCGCTATTCTGGAGAACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGctc                            <  2:475042/90‑1 (MQ=255)                                    gaACCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTacgacg              >  1:266193/1‑90 (MQ=255)                                      aCCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGt                                 <  1:347944/69‑1 (MQ=255)                                      aCCTATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGt                                 >  2:347944/1‑69 (MQ=255)                                         tATGACCACCACAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGc         >  2:368126/1‑90 (MQ=255)                                              ccaccaCAACACCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCgttgt    <  1:368126/90‑1 (MQ=255)                                                accacaacaccacaacGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCGttgttg  >  2:118997/1‑90 (MQ=255)                                                        |                                                                                  AAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTGCTTGGCCCGTTGGCCTGGCTTTTAGTCGCTCTGTTGAGTACGACGCTGGCGTTGTTG  >  NZ_CP009273/1699466‑1699602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑