Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,709,042	A→G	N137D (AAC→GAC)	gstA →	glutathione transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,709,042	0	A	G	100.0%	52.2 / NA	16	N137D (AAC→GAC)	gstA	glutathione transferase GstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

GTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGAACGAGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTC  >  NZ_CP009273/1708956‑1709117                                                                                       |                                                                            gTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACg                                                                          <  2:231552/90‑1 (MQ=255)   ttCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGGGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAg                                                                        >  1:389622/1‑90 (MQ=255)   ttCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGa                                                                         <  1:313378/89‑1 (MQ=255)   ttCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGa                                                                         >  2:313378/1‑89 (MQ=255)    tCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAgg                                                                       <  1:55343/90‑1 (MQ=255)     cacaCCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAGGc                                                                      <  2:201668/90‑1 (MQ=255)         ccTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTg                                                                  >  1:311214/1‑90 (MQ=255)          ctctGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTGa                                                                 >  1:206256/1‑90 (MQ=255)           tctGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTGaa                                                                <  1:479137/90‑1 (MQ=255)                        gATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTGAAGGATGAGCACTgg                                                   >  1:109226/1‑90 (MQ=255)                        gATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTGAAGGATGAGCACTgg                                                   >  1:262617/1‑90 (MQ=255)                                                             aGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATc              <  1:108914/90‑1 (MQ=255)                                                             aGCTGGAGAAGAAGCTGCAATATGTGGACGAGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATc              <  1:26889/90‑1 (MQ=255)                                                                         aggtGCAATCTGTGGACGAGGAACTGAAGGATGAGCCCTTGATCTGCAGGCACAGATTTCCAATTGCTGATGCCTATCTGTTTACGGTTc  <  2:142318/87‑1 (MQ=255)                                                                                   tgtgGACGAGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCt                 >  1:253554/1‑65 (MQ=255)                                                                                   tgtgGACGAGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCt                 <  2:253554/65‑1 (MQ=255)                                                                                       |                                                                            GTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAGTACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGAACGAGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTC  >  NZ_CP009273/1708956‑1709117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑