Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,721,950	A→C	P345P (CCA→CCC)	nemA →	N‑ethylmaleimide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,721,950	0	A	C	100.0%	42.4 / NA	13	P345P (CCA→CCC)	nemA	N‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/6);  total (7/6)

GTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGC  >  NZ_CP009273/1721863‑1722028                                                                                        |                                                                               gTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCccca                                                                              >  1:23740/1‑90 (MQ=255)         aTGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCg                                                                      >  1:247769/1‑90 (MQ=255)                           gTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTggcg                                                    >  1:500703/1‑90 (MQ=255)                            tGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTggcgg                                                   >  1:407104/1‑90 (MQ=255)                                 ggATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCgg                                              <  2:172232/90‑1 (MQ=255)                                      gCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGc                                         >  1:25594/1‑90 (MQ=255)                                      gCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGc                                         <  2:427758/90‑1 (MQ=255)                                             cGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCg                                  <  2:317192/90‑1 (MQ=255)                                                            gCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGt                   <  1:305105/90‑1 (MQ=255)                                                                             gAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCgg                                              >  1:452391/1‑46 (MQ=255)                                                                             gAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCgg                                              <  2:452391/46‑1 (MQ=255)                                                                             gAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAgc  >  1:222129/1‑90 (MQ=255)                                                                                    aCCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCgg                                              <  1:16169/39‑1 (MQ=255)                                                                                    aCCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCgg                                              >  2:16169/1‑39 (MQ=39)                                                                                        |                                                                               GTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGC  >  NZ_CP009273/1721863‑1722028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑