| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 204,438 | A→G | Q942Q (CAA→CAG) | dnaE → | DNA polymerase III subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 204,438 | 0 | A | G | 94.1% | 50.5 / ‑3.3 | 17 | Q942Q (CAA→CAG) | dnaE | DNA polymerase III subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (6/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
AAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACACCCTATCAACCAGTAT > NZ_CP009273/204352‑204519 | aaaGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCg < 1:406777/90‑1 (MQ=255) gAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCg < 1:235199/90‑1 (MQ=255) cTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGc < 2:274700/90‑1 (MQ=255) aTCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAg > 2:423578/1‑90 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAggt < 1:512344/90‑1 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAggt < 2:411109/90‑1 (MQ=255) atatGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATg > 2:87148/1‑90 (MQ=255) gCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTa < 1:343299/90‑1 (MQ=255) aaGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGcc < 1:4428/90‑1 (MQ=255) ttGAACAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGac < 1:423578/90‑1 (MQ=255) aCAATCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACAccc < 2:17126/90‑1 (MQ=255) aTCCTACGCCAGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACACCCTAt < 1:106739/90‑1 (MQ=255) aGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGtt < 2:348859/53‑1 (MQ=255) aGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGtt > 1:348859/1‑53 (MQ=255) aGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACACCCTATCAACCAGTAt > 1:434778/1‑90 (MQ=255) aGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACACCCTATCAACCAGTAt > 2:359068/1‑90 (MQ=255) aGCTGCCAGCCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACACCCTATCAACCAGTAt > 2:46973/1‑90 (MQ=255) | AAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTAGATGGGGAACGTGAAACGTTAGGCCTGTACCTGACCGGACACCCTATCAACCAGTAT > NZ_CP009273/204352‑204519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |