Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,751,148	A→G	A398A (GCA→GCG)	pykF →	pyruvate kinase PykF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,751,148	0	A	G	100.0%	27.5 / NA	9	A398A (GCA→GCG)	pykF	pyruvate kinase PykF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/6);  total (3/6)

GGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGA  >  NZ_CP009273/1751079‑1751234                                                                      |                                                                                       ggTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAAc                                                                    <  1:252493/90‑1 (MQ=255)        aCTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCtcat                                                             <  2:278222/90‑1 (MQ=255)             gggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCtcat                                                             >  1:177472/1‑85 (MQ=255)             gggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCtcat                                                             <  2:177472/85‑1 (MQ=255)                       tCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGc                                              >  2:188923/1‑90 (MQ=255)                               cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGt                                      <  1:188923/90‑1 (MQ=255)                                               ttCCCGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGtt                      >  2:331425/1‑90 (MQ=255)                                                   cGGATGCCACCATCCTGGCGCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAag                  <  2:485785/90‑1 (MQ=255)                                                                   ggCGCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtga  <  1:322968/90‑1 (MQ=255)                                                                      |                                                                                       GGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGA  >  NZ_CP009273/1751079‑1751234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑