Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,794,866	C→T	A10A (GCG→GCA)	infC ←	translation initiation factor IF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,794,866	0	C	T	100.0%	34.8 / NA	12	A10A (GCG→GCA)	infC	translation initiation factor IF‑3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTC  >  NZ_CP009273/1794779‑1794950                                                                                        |                                                                                     cAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGgcgtgc                                                                                    <  1:484200/90‑1 (MQ=255)   gACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCg                                                                                  >  1:29369/1‑90 (MQ=255)                 aGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGtttc                                                                    >  1:470403/1‑89 (MQ=255)                           cTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTaa                                                          <  2:182942/90‑1 (MQ=255)                                    ctcttAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTAtt                                                 <  2:274457/87‑1 (MQ=255)                                    ccgTTAGGCGACCTCCCTGGCGGCGAATTTCTACATTAATCCGTTCAGGGCGTGCCGTTTGATTTCGTTTCCCGCCTTTAATACCTTAtt                                                 <  2:334434/88‑1 (MQ=255)                                            cGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAAt                                         <  2:405177/90‑1 (MQ=255)                                                cTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGtt                                     >  1:273409/1‑90 (MQ=255)                                                          gcgAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCg                           >  2:214726/1‑90 (MQ=255)                                                                  cGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGaa                   <  2:472790/90‑1 (MQ=255)                                                                   gCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAAt                  <  1:481520/90‑1 (MQ=255)                                                                                 tAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCt    <  2:88284/90‑1 (MQ=255)                                                                                   gggCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTtc  >  1:153952/1‑90 (MQ=255)                                                                                        |                                                                                     CAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTC  >  NZ_CP009273/1794779‑1794950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑