Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,794,884	T→C	G4G (GGA→GGG)	infC ←	translation initiation factor IF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,794,884	0	T	C	100.0%	43.4 / NA	14	G4G (GGA→GGG)	infC	translation initiation factor IF‑3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/9);  total (5/9)

CTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTT  >  NZ_CP009273/1794805‑1794960                                                                                |                                                                             cTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTaa                                                                    <  2:182942/90‑1 (MQ=255)          ctcttAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTAtt                                                           <  2:274457/87‑1 (MQ=255)          ccgTTAGGCGACCTCCCTGGCGGCGAATTTCTACATTAATCCGTTCAGGGCGTGCCGTTTGATTTCGTTTCCCGCCTTTAATACCTTAtt                                                           <  2:334434/88‑1 (MQ=255)                  cGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAAt                                                   <  2:405177/90‑1 (MQ=255)                      cTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGtt                                               >  1:273409/1‑90 (MQ=255)                                gcgAATTTCGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCg                                     >  2:214726/1‑90 (MQ=255)                                        cGCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGaa                             <  2:472790/90‑1 (MQ=255)                                         gCCATTGATACGGTTAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAAt                            <  1:481520/90‑1 (MQ=255)                                                       tAGGGCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCt              <  2:88284/90‑1 (MQ=255)                                                         gggCGTGCCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTtc            >  1:153952/1‑90 (MQ=255)                                                             gtgcCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGa        >  2:384531/3‑90 (MQ=255)                                                               gcCGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATc      <  1:214726/90‑1 (MQ=255)                                                                 cGTTTGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCAc    <  2:96612/90‑1 (MQ=255)                                                                   tttGAACTCGTTTCCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACtt  >  2:367634/1‑90 (MQ=255)                                                                                |                                                                             CTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTT  >  NZ_CP009273/1794805‑1794960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑