Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,803,183	T→C	D103G (GAT→GGT)	yniB ←	YniB family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,803,183	0	T	C	100.0%	57.2 / NA	17	D103G (GAT→GGT)	yniB	YniB family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

TCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGG  >  NZ_CP009273/1803096‑1803265                                                                                        |                                                                                   tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAAccc                                                                                  >  2:202359/1‑90 (MQ=255)          ttGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGcc                                                                         >  2:40329/1‑90 (MQ=255)            ggCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAg                                                                       >  1:118886/1‑90 (MQ=255)                                     tCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATc                                              >  1:420291/1‑90 (MQ=255)                                       aaCACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAc                                            >  2:98317/1‑90 (MQ=255)                                          acCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCa                                         <  2:38488/90‑1 (MQ=255)                                           ccTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAg                                        <  1:277147/90‑1 (MQ=255)                                             ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt                                               >  1:363516/1‑81 (MQ=255)                                             ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt                                               >  1:506532/1‑81 (MQ=255)                                             ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt                                               <  2:506532/81‑1 (MQ=255)                                             ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt                                               <  2:363516/81‑1 (MQ=255)                                             ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGaa                                      >  2:54229/1‑90 (MQ=255)                                                ccGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACAt                                   <  2:37829/90‑1 (MQ=255)                                                 cGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAACCCTACAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                                  <  1:329940/90‑1 (MQ=255)                                                         gcgcgcCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCAc                          >  1:16043/1‑90 (MQ=255)                                                            cgcCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                       <  1:239014/90‑1 (MQ=255)                                                             gcCTGGCGACTCATCCGGGCACCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                      <  1:85320/90‑1 (MQ=255)                                                                                 aCCGGAACCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCgg  <  1:202359/90‑1 (MQ=255)                                                                                        |                                                                                   TCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGG  >  NZ_CP009273/1803096‑1803265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑