| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,840,050 | T→C | S390G (AGT→GGT) | topB ← | DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,840,050 | 0 | T | C | 84.2% | 41.5 / 1.9 | 19 | S390G (AGT→GGT) | topB | DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (8/8); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
ATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGC > NZ_CP009273/1839978‑1840138 | aTTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAatga < 1:377342/90‑1 (MQ=255) gCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATgg > 2:316543/1‑90 (MQ=255) ggCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGAc > 2:489123/1‑90 (MQ=255) gTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTc < 1:447873/90‑1 (MQ=255) ataGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATc < 1:489123/90‑1 (MQ=255) cttcgcttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGa > 1:477472/1‑49 (MQ=255) cttcgcttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGa < 2:477472/49‑1 (MQ=255) cttcgcttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACa > 1:454852/1‑90 (MQ=255) cttcgcttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACa > 1:216555/1‑90 (MQ=255) tcgcttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGc > 1:261393/1‑90 (MQ=255) cttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGt < 1:477341/90‑1 (MQ=255) ttcgTTCTCCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGtt < 2:261393/90‑1 (MQ=255) ctcCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCgt < 1:354885/47‑1 (MQ=255) ctcCGTCAGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCgt > 2:354885/1‑47 (MQ=255) aGGTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGa < 2:454852/90‑1 (MQ=255) ggTTGATCGCAGAACCCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGAt > 2:34649/1‑90 (MQ=255) gATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATc > 1:380055/1‑87 (MQ=255) gATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATc < 2:380055/87‑1 (MQ=255) cTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGc > 2:182825/1‑90 (MQ=255) | ATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGC > NZ_CP009273/1839978‑1840138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |