Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,876,783	A→G	R205R (CGA→CGG)	dmlA →	multifunctional D‑malate/3‑isopropylmalate/tartarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,876,783	0	A	G	100.0%	66.4 / NA	19	R205R (CGA→CGG)	dmlA	multifunctional D‑malate/3‑isopropylmalate/tartarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

CTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAG  >  NZ_CP009273/1876724‑1876867                                                            |                                                                                     cTAACTTCTGCCACTAAATCGAACGGTTTAGCCGTCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGgag                                                        >  2:87396/1‑90 (MQ=255) cTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGgag                                                        >  1:65989/1‑90 (MQ=255)       tCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGc                                                  <  2:65989/90‑1 (MQ=255)       tCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGc                                                  <  2:256142/90‑1 (MQ=255)              cTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACa                                           <  2:435958/90‑1 (MQ=255)                        cGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCatat                                 <  2:199353/90‑1 (MQ=255)                        cGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCatat                                 <  2:211288/90‑1 (MQ=255)                          gTTTAGCCATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTg                               >  2:520448/1‑90 (MQ=255)                                 cATCAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATtct                        <  1:521112/90‑1 (MQ=255)                                    cAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTAccc                                                            >  1:258216/1‑51 (MQ=255)                                    cAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTAccc                                                            <  2:258216/51‑1 (MQ=255)                                    cAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGgaga                                                       <  2:89657/56‑1 (MQ=255)                                    cAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGgaga                                                       >  1:89657/1‑56 (MQ=255)                                    cAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTg                     >  1:158256/1‑90 (MQ=255)                                    cAGCATGCCGTACTGGGATGAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTg                     >  1:121157/1‑90 (MQ=255)                                                       gAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAg  >  2:151180/1‑90 (MQ=255)                                                       gAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAg  >  2:430887/1‑90 (MQ=255)                                                       gAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAg  >  1:5068/1‑90 (MQ=255)                                                       gAGCGGGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAg  >  1:386872/1‑90 (MQ=255)                                                            |                                                                                     CTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAG  >  NZ_CP009273/1876724‑1876867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑