Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,880,952	A→G	E284G (GAA→GGA)	yeaX →	carnitine monooxygenase subunit YeaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,880,952	0	A	G	100.0%	61.7 / NA	18	E284G (GAA→GGA)	yeaX	carnitine monooxygenase subunit YeaX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

GGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATC  >  NZ_CP009273/1880869‑1881022                                                                                    |                                                                       ggTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGa                                                                  >  2:385978/1‑90 (MQ=255)        gAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGAc                                                           <  2:142252/90‑1 (MQ=255)                taaAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGa                                                   <  1:90906/90‑1 (MQ=255)                       gcgAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAatat                                            >  1:191354/1‑90 (MQ=255)                       gcgAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAatat                                            >  2:390687/1‑90 (MQ=255)                            aGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAg                                       <  1:368063/90‑1 (MQ=255)                            aGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAg                                       <  2:166544/90‑1 (MQ=255)                                      ttATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAagag                             <  1:404605/90‑1 (MQ=255)                                                     gTATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCaa              <  1:390687/90‑1 (MQ=255)                                                      tATGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGCCCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCaaa             <  2:456382/90‑1 (MQ=255)                                                       aTGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCaa              >  2:489606/1‑88 (MQ=255)                                                       aTGCGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCaa              <  1:489606/88‑1 (MQ=255)                                                          cGGAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTAt         <  1:425555/90‑1 (MQ=255)                                                            gAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGt       >  2:89802/1‑90 (MQ=255)                                                            gAACCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGt       >  1:74675/1‑90 (MQ=255)                                                              aCCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTg     >  1:262851/1‑90 (MQ=255)                                                              aCCTGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTg     >  1:20889/1‑90 (MQ=255)                                                                 tGCGAAACAGCAATACTGGGAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATc  >  2:156252/1‑90 (MQ=255)                                                                                    |                                                                       GGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATC  >  NZ_CP009273/1880869‑1881022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑