| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 371,042 | 0 | A | C | 58.8% | ‑0.2 / 15.8 | 17 | Q43P (CAG→CCG) | mhpT | 3‑(3‑hydroxy‑phenyl)propionate transporter |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base C (10/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGC > NZ_CP009273/370959‑371112 | gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCttt > 1:560814/1‑90 (MQ=255) aCCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCtttt > 1:205312/1‑89 (MQ=255) aTCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGCATCGCCCCGGCTTTTGCa > 1:88049/1‑90 (MQ=255) tCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGGTTTCGccc > 2:380920/1‑88 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGGGTCGCCCCGGCTTTCGCACTCGATaa > 1:562305/1‑90 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGGTCGCCCCGGCTTTTGCCCCCGATaa > 2:22113/1‑90 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCAGGTGGCATCGCCCAGGCTTTCGCACTCGATaa > 1:167220/1‑90 (MQ=255) cGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCTTTCGCACTCGATAAAATGCAAATggg > 1:350570/1‑90 (MQ=255) ctctGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCt < 1:457093/90‑1 (MQ=255) aaGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGCATCGCCCCGGCTTTTGCACTCGATAAAATGCAAAAGGGGTGGGTATTTa > 1:137604/1‑90 (MQ=255) ggCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCTTTTTCCCTCGCTAAAAAGCAAAAGGGGTGGGTAtttttcg > 2:359788/1‑86 (MQ=255) gCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAgcgc < 1:242284/90‑1 (MQ=255) ttCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCCGGCTTTCGCACTCGATAAAATGCAAATGGGGTGGGTATTTAGCGCCGGAATAc > 1:50381/1‑90 (MQ=255) tCAGGCGGCTGGCATTGCGGCGGGGGGCATCGCCCAGGCCTTTGCACTCGATAAAATTCAAATGGGGTGGGTATTTAGCGCCGGAAAACt > 2:286986/1‑90 (MQ=255) cAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTc < 2:205312/90‑1 (MQ=255) ggcggcTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCgg < 1:169456/90‑1 (MQ=255) gcggcgGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGgc < 2:192527/90‑1 (MQ=255) | GACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGC > NZ_CP009273/370959‑371112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |