| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,134,407 | 0 | A | C | 57.1% | 7.8 / 14.0 | 14 | G229G (GGT→GGG) | asmA | outer membrane assembly protein AsmA |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGGTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGAT > NZ_CP009273/2134319‑2134491 | tttGGTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCAc < 2:93653/90‑1 (MQ=255) gTTAATTGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTg > 1:424301/1‑90 (MQ=255) gTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTaa > 1:536446/1‑90 (MQ=255) gTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTaa > 2:123038/1‑90 (MQ=255) tcccACTGGGCCTGAAACCCCCCCTGCCCCTTAATCCCTTTTTTTGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTCAATAgcc < 2:242020/88‑1 (MQ=255) cTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGa > 1:22303/1‑90 (MQ=255) gcccctgAATACCTTGTTTTGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAg < 1:123038/90‑1 (MQ=255) tGTTTTGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTTTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgta < 1:12035/90‑1 (MQ=255) tttttGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgtac < 1:135115/89‑1 (MQ=255) tttttGGAAAACGGGCCCCCTGCAACTGCCAATTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgtac < 2:499738/89‑1 (MQ=255) ttttGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTAAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgtacc < 1:485919/90‑1 (MQ=255) ttttGGTAAATCGCCCCCCTCCACCCGCAATTTATTTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgtacc < 2:611100/90‑1 (MQ=255) aaTCGGCCCCCTGCAACTGCCATTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAggg < 1:302904/90‑1 (MQ=255) tCGGCACCCTGCAACTGCCAGTTATTTTTTTAAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGat < 2:536446/90‑1 (MQ=255) | TTTGGTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGAT > NZ_CP009273/2134319‑2134491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |