Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,175,9570AT57.1% 5.1 / 20.6 21I17F (ATT→TTT) zupTzinc transporter ZupT
Reads supporting (aligned to +/- strand):  ref base A (3/6);  new base T (12/0);  total (15/6)
Fisher's exact test for biased strand distribution p-value = 1.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGC  >  NZ_CP009273/3175886‑3176044
                                                                       |                                                                                          
tGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCg                                                                         <  1:114789/90‑1 (MQ=255)
 gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGGTTTTTTGCGCGGTTCTCTGCgg                                                                        >  2:369543/1‑89 (MQ=255)
 gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGGTTCTCGGccg                                                                        >  1:600928/1‑88 (MQ=255)
       ttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTTGGGCGGTTTTCGGCGTTCTCgg                                                                 >  1:206029/1‑90 (MQ=255)
                gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTGGCGCGTTTTTCGGCGTTCTCGGGCaaaaaac                                                       >  1:184412/1‑88 (MQ=255)
                   gTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGGTTTTTTGGGCGGTTTTCCGCGTTCTCGGGCCAAAAAcccc                                                     >  2:439978/1‑89 (MQ=255)
                               aCCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTGGCGCGGTTTTCCGCGTTCTCCGGCAAAAACCCCCGAACCCCCTACt                                        >  1:453752/1‑90 (MQ=255)
                                  tctcATTCTGACCATACTGGCGGGGGGCGCCACGTTTTTTGGCGCGGTTTTCGGCGTTCTCCGGCAAAAAACCTCGAACCGCCTTCTGGc                                     >  1:165469/1‑90 (MQ=255)
                                  tctcATTCTGACCATACTGGCGGGGGGAGCCACGCTTTTTTGTGCGTTTTTCTGCCCTTTTCGGGCAAAACCCCCCCAACCCTTACCGGc                                     >  2:326008/1‑90 (MQ=255)
                                  tctcATTCTGACCATACTGGCGGGGGCAGGCACGCTTATTTTCGCGCTTTTCTGCGTTCTCGGGGGAAAAACCCCCAACCGCCTTCTGGc                                     >  2:376871/1‑90 (MQ=255)
                                     cATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGtt                                  <  2:282444/90‑1 (MQ=255)
                                           gACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCCCGAACCGCTTACTGGCGGTTTCGCt                            >  1:13741/1‑90 (MQ=255)
                                             ccATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCGGCGTTCTCGGGCAAAAAACCCCGATCCGCTTTCTGGCGTTTTTGCTAg                          >  1:577920/1‑90 (MQ=255)
                                               aTACTGGCGGGGGGAGCCACGTTTTTTGGGGCGGTTGTCCGCGTTCTTCGGGAAAAAACCCCGCACCGCTTTCTTGCGTTTTTGCCAggg                        >  2:183018/1‑89 (MQ=255)
                                               aTACTGGCGGGGGCAGCCACGTTTTTTGGCGCGGTTTTCGGCGTTCTCGGGCAAAAAACCCCGAACCCCTTTCTGGCGGTTTTGCTAggg                        >  2:199538/1‑89 (MQ=255)
                                               aTACTGGCGGGGGCAGCCACGTTTATTTGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCCCGAACCCCTTACTGGCGGTTTTGCTAGGt                        >  1:269107/1‑90 (MQ=255)
                                                     gCGGGGGCAGCCACGGTTATTGGCGCGTTTTTTTGCGTTCTCCGGCAAAAAACCCCCCACCCCATACTTGCGGTTTTGCTCTGtttcttt                  >  2:391104/1‑86 (MQ=255)
                                                        ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg               <  2:192303/90‑1 (MQ=255)
                                                             aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt          <  1:183018/90‑1 (MQ=255)
                                                               agaCGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCa        <  2:80310/88‑1 (MQ=255)
                                                                     ttATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGc  <  2:600928/90‑1 (MQ=255)
                                                                       |                                                                                          
TGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGC  >  NZ_CP009273/3175886‑3176044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: