| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,544,923 | 0 | C | A | 53.8% | 14.9 / 45.2 | 26 | N177K (AAC→AAA) | uxuR | Uxu operon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (1/11); new base A (13/1); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCA > NZ_CP009273/4544834‑4544994 | gACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAc < 1:128544/90‑1 (MQ=255)gACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAc < 2:606570/90‑1 (MQ=255)gACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAc < 2:453077/90‑1 (MQ=255) gCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATcc < 2:370754/90‑1 (MQ=255) gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAATGTGGATGCAg > 1:111380/1‑90 (MQ=255) tGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAATGTTGGTGCCGTTGcc > 1:424375/1‑89 (MQ=255) gCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGcac < 1:610719/90‑1 (MQ=255) gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAAACAAAGTGGGTCCAGGTGcacaac > 2:6722/1‑88 (MQ=255) gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGc < 2:23615/90‑1 (MQ=255) aGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCa < 1:88406/90‑1 (MQ=255) aGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGATCCAGTTGCACAGCCa < 2:336677/90‑1 (MQ=255) gacagCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATc < 2:581385/86‑1 (MQ=255) acaggCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGAc < 2:314052/86‑1 (MQ=255) aCAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTGGGTCCAGTTGCACAGCCCACTGGGTggaa > 2:49059/1‑87 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAAATTGGGTCCCGTTGCACCGCCACCTGGGTGGCAAcc > 2:363219/1‑90 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAt < 2:612865/55‑1 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGcc < 1:267612/90‑1 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAAt > 1:612865/1‑55 (MQ=255) aTGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATACAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTg > 1:206632/1‑90 (MQ=255) aTGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCATGTGGGTCCAGTTGCACAGCCCTCTGGGTGGCAGcccg > 2:208096/1‑88 (MQ=255) tGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCCATGTGGGTCCCGTTTCACAGCCCTCTTGGTGACAGccgtt > 1:581385/1‑87 (MQ=255) ggtggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAAAGTGGGTCCCGGTGTGCCACCACCTTGGTGACAGCCTGGAttg > 1:415252/1‑88 (MQ=255) gtggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGGTCCAGTTGCACAGCCCTCTGGATGACAGCCTGTATCGc > 1:224637/1‑90 (MQ=255) gtggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGTCCAGTTGCACAGCCATCTGGGTGACAGCCCGTATCGc > 1:175/1‑90 (MQ=255) aGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAag < 2:102548/90‑1 (MQ=255) tGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTGGGTCCAGTTGCACCGCCCTCTGGGGGACAACCCGCATCGCCAagagg > 2:628979/1‑89 (MQ=255) ttCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGATCCCGTTGGACAGCCCTCTGGATGACAGCCCGTATCGCAAAGAgggg > 1:102548/1‑90 (MQ=255) cAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGc < 1:61642/90‑1 (MQ=255) cTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATca < 1:162860/90‑1 (MQ=255) | GACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCA > NZ_CP009273/4544834‑4544994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |