Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273228,1810CT53.8% 2.6 / 13.3 13F191F (TTC→TTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base T (7/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGT  >  NZ_CP009273/228094‑228253
                                                                                       |                                                                        
gggCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCaa                                                                        <  1:136191/90‑1 (MQ=255)
    gTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAATAcc                                                                    >  2:442913/1‑90 (MQ=255)
       gATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTgg                                                                 <  1:442913/90‑1 (MQ=255)
        aTGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGa                                                                <  2:144187/90‑1 (MQ=255)
                 gcaaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGaa                                                       <  2:630072/88‑1 (MQ=255)
                   aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTTATGCCTGGGGGCGGAGAAgg                                                     >  2:142019/1‑90 (MQ=255)
                    aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGAGTTTAAAACCCGGAGGCGGAGaaagg                                                    >  2:699949/1‑87 (MQ=255)
                        gTTACTTCCTATTGGCGATCAGATTGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTCAATGCCTGGGGCCGGAGAAGGATGaa                                                >  1:81665/1‑90 (MQ=255)
                                  aTTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATTGCCGGGGGCGGGGAAGGATGAAAGCGGTttat                                      >  1:368532/1‑90 (MQ=255)
                                        gATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAAAGCCCGGGGCCGGGGAAGGGGGAAAGCGGTTTATTGCttt                                >  2:195099/1‑90 (MQ=255)
                                          tCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAAATCCCGGGGCCGGAGAAGGGGGAAAGCGGTTTATTGCttttg                              >  2:566270/1‑88 (MQ=255)
                                                           gCGGCCCGGTCATTATGGCGGGAGGTTTTAATTCCTGGGGCCGGAGAAGGATGAACGCGGTATATTGCTTTTCGCGGGGAAAGTCGCTgc             >  1:530117/1‑90 (MQ=255)
                                                                      aTTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGt  <  1:212412/90‑1 (MQ=255)
                                                                                       |                                                                        
GGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGT  >  NZ_CP009273/228094‑228253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑