| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,931 | 0 | A | C | 56.2% | 3.5 / 17.1 | 16 | T138P (ACA→CCA) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base C (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGA > NZ_CP009273/3012870‑3013013 | cGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCCTTTCGCGACG‑CATTTTTTAAAATTgg > 1:261883/1‑90 (MQ=255) ggATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGGGCTCCCCACTTCCCGACGCCCTTTTTAAAATTTgc > 2:445675/1‑89 (MQ=255) tCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCCCCCCCCCCTTCGCGGCG‑CCTTTTTTTAAATTTGGCTTcgcgcg > 2:205380/1‑90 (MQ=255) tATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCGTTCCCCAATGCGCGACG‑CATTTTTTAAAAATGGGCTGCGCCCGCTGag > 1:388096/1‑89 (MQ=255) aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc < 2:261883/90‑1 (MQ=255) cACCATGCCTCTCCGGCGTATATCGGCGGGGGGGCCTCCCCCTTTCGCGGCC‑CATTTTTTTAAATTTGTCCGCGGGCGGTGGCCCGtttt > 2:505039/1‑90 (MQ=255) aTGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCCCATTGCGCGGCG‑CCTTTTTTAAAATTGGGCTGCGCGCGGTGGCCTGttttttaa > 1:128928/1‑86 (MQ=255) aTGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCAATTCGCGACG‑CATTTTTTAAAAATGGCCTGCGCGCGGTGACCCGttttttaa > 1:174115/1‑86 (MQ=255) ctctCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAactac < 2:191060/90‑1 (MQ=255) ctcCGGCGTATATCGGCGGGGCGCGCTCCCCCATGCCCGACGCCCTTTTTAAAATTTGG‑CTGCGGGCCGGGGCCTGTTTTTATaatactt > 2:344675/1‑89 (MQ=255) cGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGAcc < 2:175132/90‑1 (MQ=255) gTATATCGGCGGGGGGCTCTCCCCCTTTCGCGGCGCCTTTTTTTAAATTTGGCC‑GCGCGCGGGGACCCGTTTTTTAAATACTGACCCTaa > 2:496208/1‑90 (MQ=255) atCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTaacaac < 2:388096/90‑1 (MQ=255) cggcggGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACgg < 2:174115/90‑1 (MQ=255) gTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCaa < 1:97074/90‑1 (MQ=255) gCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAaga > 2:567406/1‑90 (MQ=255) | CGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGA > NZ_CP009273/3012870‑3013013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |