| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 52.9% | 5.6 / 21.0 | 17 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (1/7); new base T (8/1); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCA > NZ_CP009273/3175870‑3176038 | aCGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTAtt < 2:241510/90‑1 (MQ=255) tCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGGCACGTTTTTTgggg > 2:580745/1‑88 (MQ=255) tCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGCTTTTTTgcg > 2:61912/1‑90 (MQ=255) aGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTGGCGCGTTTTTCGGCGTTTTCGGGCAAAAAc > 1:64423/1‑90 (MQ=255) acaggTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGa < 2:436340/86‑1 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTGGGGCGTTTTTTCGCGGTTTCGTGCAAAAAACCCCGAAcc > 2:101731/1‑90 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCGGCGTTCTCGGGGAAAAAACCTCGAAACGCttt > 1:197552/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGGTTCTCCGCGTTCTCGGGCAAAAAACCCCGAACCGCCTTCTGGc > 1:54868/1‑90 (MQ=255) tGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGc < 2:307418/90‑1 (MQ=255) ggACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGc < 2:76146/89‑1 (MQ=255) gACCATACTGGCGGGGGCAGCCACGTTTTTTTGGGCGTTTTTCGGCGTTCTCGGGCAAAAACCCCCCAACCGCTTACTGGCGGTTTTGCt > 1:314071/1‑90 (MQ=255) aTACTGGCGGGGGGAGCCCCGGTTTTTGGCGCGGTTTTTGGCGTTCTCGGGCAAAAAACCCCGAACCCCTTTCTGGCGTTTTTGCTTggg > 2:26130/1‑89 (MQ=255) aTACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGt < 1:61912/90‑1 (MQ=255) gCGGGGGCAGCCACGTTTATTGGCGCGTTTTTCGGGGGTTTCGGGCAAAAAACCTCGAACCGCTTTTCGGGGTTTTTGCTAGGtttttcc > 1:76146/1‑87 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:26130/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:14467/90‑1 (MQ=255) ccACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCa < 2:54868/90‑1 (MQ=255) | ACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCA > NZ_CP009273/3175870‑3176038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |