| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,634,341 | 0 | C | T | 52.6% | 11.4 / 26.0 | 19 | F40F (TTC→TTT) | dtpB | dipeptide/tripeptide permease DtpB |
| Reads supporting (aligned to +/- strand): ref base C (1/8); new base T (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTG > NZ_CP009273/3634252‑3634429 | cagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc < 1:27433/90‑1 (MQ=255) gcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttctt < 2:82689/90‑1 (MQ=255) cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTtaa > 2:27530/1‑90 (MQ=255) tGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCa < 2:59941/90‑1 (MQ=255) tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTg < 1:27530/90‑1 (MQ=255) gATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTg < 1:72384/90‑1 (MQ=255) ggCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGCTTGGGTTTTCGCCAAAGGAGGGTTTTTTCACTTTTtgtggt > 1:422399/1‑88 (MQ=255) ggCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCt < 1:588777/90‑1 (MQ=255) tactacGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCtttt < 1:414010/90‑1 (MQ=255) ctacGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTtgc < 2:17886/90‑1 (MQ=255) gCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAACTTGGATTTTCGCAAAAGGAGGGTTTTTTTACTTTTTGTGGTTTTTCTgcgc > 1:576606/1‑90 (MQ=255) gCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTAAACAACTTGGATTTTCGCAAGAGCAGGCTTTTTTCACTTTTTGTGCTTTTTCTgcgc > 1:491721/1‑90 (MQ=255) gTGCAGGGCGTACTGGCGGTTTTTTTTCTTTAAAAACTTGGGTTTTCGCAAGGGCAGGGTTTTGTTACTTTTTGTGGTTTTTCTGCGCTg > 2:256259/1‑90 (MQ=255) gCAGGGCGTACTGGCGGGTTTTTTCGTTTAAAAACTTGGGTTTTCGCCAGAGCAGGCTTTTGTCACTTTTTGTGGTTTTTTTGCGCTGGt > 2:166203/1‑90 (MQ=255) cAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAACTTGGGTTTTCGCCAGAGGAGGCTTTTTTTTCTTTTTGTGCTTTTTCTGCGCTGGTc > 2:312569/1‑90 (MQ=255) gggCGTACTGGCGGTTTTTTTCGTTAAACAGCTTGGGTTCTCGCAAGAGCAGGCTTTTGTTACTTTTTGTGCTTTTTCTGCGCTGGGCtt > 2:270313/1‑89 (MQ=255) gCGTACTGGCGGTTTTTTTCGTTAAAAAGCTTGGGTTTTCGCAAGAGCGGGCTTTTTTTACTTTTTGTGCTTTTTCTGCGCTGGGCTATg > 2:524876/1‑90 (MQ=255) tACTGGCGGTTTTTTTCGTTAAACAGCTTGGATTCTCGCAAAAGCAGGCTTTTTTCACTTTTTGTGCTTTTTCTGCGCTGGGCTATGGcc > 2:191232/1‑90 (MQ=255) cTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTc < 1:8999/90‑1 (MQ=255) gCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCt < 1:563421/63‑1 (MQ=255) gCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCt > 2:563421/1‑63 (MQ=255) tcttcGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTg < 1:295055/90‑1 (MQ=255) | CAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTG > NZ_CP009273/3634252‑3634429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |