| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,734,269 | 0 | T | G | 37.8% | 55.9 / 12.2 | 37 | V402G (GTG→GGG) | avtA | valine‑‑pyruvate transaminase |
| Reads supporting (aligned to +/- strand): ref base T (7/16); new base G (14/0); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734192‑3734358 | ggATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGc > 2:108745/1‑90 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 1:108745/90‑1 (MQ=255) aCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAaga > 1:66016/1‑90 (MQ=255) gTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGAt < 2:66016/90‑1 (MQ=255) gTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGAt < 2:400013/90‑1 (MQ=255) tGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATa > 1:436793/1‑90 (MQ=255) cGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaaa < 2:570291/90‑1 (MQ=255) aCGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGcc < 2:171987/90‑1 (MQ=255) cATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAATTTGGCGGGAGAGGTAGAAAGAGGCTgg > 2:556472/1‑90 (MQ=255) aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGa < 2:569911/67‑1 (MQ=255) aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGa > 1:569911/1‑67 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGATAGAAAAAGCCTGGGCTGAAAGTc > 2:370740/1‑90 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAAAGCCTGGGCTGAAAggc > 1:287300/1‑88 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAGTCTGGTGGAAGAGATAGAAAGAGCCTGGGCGGAAAgac > 2:297496/1‑88 (MQ=255) cgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGGGGAAGAGATAAAAAGAGCCTGGGGTGAAAGtttc > 1:72037/1‑87 (MQ=255) aTGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTaa > 2:132861/1‑90 (MQ=255) aTGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTaa > 1:164774/1‑90 (MQ=255) gAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGGAGATTTTGGGGGGAGAGATAGAAAAAGCCTGGGGTGAAAGTCACTaaaa > 2:294832/1‑90 (MQ=255) aCGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGc > 1:240037/1‑90 (MQ=255) aCGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGc > 1:161658/1‑90 (MQ=255) cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGcc > 1:1269/1‑90 (MQ=255) cAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGATAGAAAAAGCCTGGGGTGAAAGGCACTAAAAAATGCCTGATg > 2:127151/1‑90 (MQ=255) agagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 1:476106/44‑1 (MQ=255) agagCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAag > 2:476106/1‑44 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCt < 2:353887/84‑1 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCt > 1:353887/1‑84 (MQ=255) gCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCt > 2:126904/1‑90 (MQ=255) cGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTAc < 1:549620/90‑1 (MQ=255) ggAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTg > 1:427689/1‑76 (MQ=255) ggAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTg < 2:427689/76‑1 (MQ=255) ggAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg > 1:487081/1‑90 (MQ=255) gagaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGc < 1:294832/90‑1 (MQ=255) gagaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGc < 1:132861/90‑1 (MQ=255) gagaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGc < 1:317135/90‑1 (MQ=255) gagaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGc < 2:487081/90‑1 (MQ=255) aaTTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATc < 1:423337/90‑1 (MQ=255) gggggTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa < 1:126904/90‑1 (MQ=255) tGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAtt < 1:330628/90‑1 (MQ=255) | GGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734192‑3734358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |