| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,187 | 0 | G | C | 57.1% | 6.7 / 18.4 | 14 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base C (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG > NZ_CP009273/760116‑760270 | gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGGTCTAACTTTTTCGAc > 1:72108/1‑90 (MQ=255)gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGGCCCCTCCCCGGCTCTAAATTTTTctcc > 2:134409/1‑87 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGt < 2:276352/90‑1 (MQ=255) cGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGa < 1:477975/90‑1 (MQ=255) ttCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCCGGCTCTAACTTTTTCGACATTTTCGAAATGTTTGAAAAAAAACCg > 1:72114/1‑90 (MQ=255) cGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCa < 1:109515/90‑1 (MQ=255) gTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTCGACATTCTCCAAAAGTTCGAAAAAAAACCCCAAACCGa > 1:566516/1‑90 (MQ=255) gtgtCGGTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTTGACATTCTCGAAAAGTTTGAAAAAAAACCGCCGACCGAAACGGTCg > 1:365544/1‑90 (MQ=255) gtgtCGGTATCGGCGGGGGCCCCCTCCCCGGGGCTCACTTTTTTTACATTTTCGGAAAGTTTGGAAAAAATACGCCGACCCAAGCGATCg > 2:273771/1‑90 (MQ=255) ggTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGtgatg < 1:140933/90‑1 (MQ=255) gTATCGGCGGTGACCCGATCCCCGGCTCTAACTTTTTTTACATTTTCGAAAAGTTTGAAAAAAAACCGCAGACCGAAGCGGGCGtgaaga > 1:275397/1‑90 (MQ=255) cggcggTGACCCCATCCCCGGCTCTAACTTTATTGACATTTTCGAAATGTTCGAAAAAAAACCGCAGACCGAAGCGATCGTGATGATCgg > 1:242950/1‑90 (MQ=255) gATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcgg > 1:234496/1‑90 (MQ=255) gATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcgg > 1:467355/1‑90 (MQ=255) | GCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG > NZ_CP009273/760116‑760270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |