| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,845,013 | 0 | T | G | 57.1% | 5.5 / 13.2 | 15 | G218G (GGT→GGG) | hypB | hydrogenase nickel incorporation protein HypB |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCT > NZ_CP009273/2844943‑2845096 | aCCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAat < 2:236914/90‑1 (MQ=255) agtCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAata < 1:373275/88‑1 (MQ=255) ccGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGGAAAAAAACCACTGAAAAATACGCATATg > 1:443148/1‑90 (MQ=255) ggCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAAACACTGAAAAATCCGCATATGtt > 2:64417/1‑90 (MQ=255) ggCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGGCTTTCCGTTTCCGAAGGGGAAGAAAAAACACTGAAAAATCCGCATATgct > 2:373275/1‑88 (MQ=255) gCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAAAATCCCCATATGttt > 1:337087/1‑90 (MQ=255) gCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGGAGGGGAAGAAAAAACCCTGAAAAATCCGCATATGTTTtccg > 2:392940/1‑90 (MQ=255) gATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTgccgccgcc < 1:124985/90‑1 (MQ=255) aTCTCGGTGAAAAACACAAAGTGGCGGTACTTTCCCTTTCCGAAAGGGAAAACAAACCACTTGAAAATACGCATATGTTTttccccgccc > 2:385435/1‑89 (MQ=255) cGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGAAGAAAAACCACTGAAAAATCCGCATATGTTTGCCGCCGCCTCGCt > 1:562982/1‑90 (MQ=255) cGGTGAAAAACACAAAGTGGCGGAGCTTTCCGTTTCCCAAAGAGAAGAAAAACCCCTGAAAAAACCGCATATGTTTGCCGCCCCCCCGcc > 2:124985/1‑89 (MQ=255) aaaaCACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGAtgct > 1:140257/1‑90 (MQ=255) aaaGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTcaac < 2:6919/90‑1 (MQ=255) cGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGcc < 1:483992/90‑1 (MQ=255) cGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAtct < 1:385435/90‑1 (MQ=255) | ACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCT > NZ_CP009273/2844943‑2845096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |