| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,922 | 0 | T | G | 52.6% | 18.6 / 23.9 | 19 | S135A (TCG→GCG) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAA > NZ_CP009273/3012836‑3013010 | gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGttcg > 1:191060/1‑87 (MQ=255)gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGgcgc > 1:38861/1‑90 (MQ=255)gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCgg > 2:567757/1‑89 (MQ=255) cGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGct < 2:190754/90‑1 (MQ=255) ggACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCggcc > 2:505934/1‑87 (MQ=255) aCTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCtctc < 1:235788/90‑1 (MQ=255) gCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGt > 1:125639/1‑54 (MQ=255) gCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGt > 2:313588/1‑54 (MQ=255) gCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGt < 2:125639/54‑1 (MQ=255) gCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGt < 1:313588/54‑1 (MQ=255) cGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCCTTTCGCGACG‑CATTTTTTAAAATTgg > 1:261883/1‑90 (MQ=255) ggATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGGGCTCCCCACTTCCCGACGCCCTTTTTAAAATTTgc > 2:445675/1‑89 (MQ=255) tCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCCCCCCCCCCTTCGCGGCG‑CCTTTTTTTAAATTTGGCTTcgcgcg > 2:205380/1‑90 (MQ=255) tATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCGTTCCCCAATGCGCGACG‑CATTTTTTAAAAATGGGCTGCGCCCGCTGag > 1:388096/1‑89 (MQ=255) aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc < 2:261883/90‑1 (MQ=255) cACCATGCCTCTCCGGCGTATATCGGCGGGGGGGCCTCCCCCTTTCGCGGCC‑CATTTTTTTAAATTTGTCCGCGGGCGGTGGCCCGtttt > 2:505039/1‑90 (MQ=255) aTGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCCCATTGCGCGGCG‑CCTTTTTTAAAATTGGGCTGCGCGCGGTGGCCTGttttttaa > 1:128928/1‑86 (MQ=255) aTGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCAATTCGCGACG‑CATTTTTTAAAAATGGCCTGCGCGCGGTGACCCGttttttaa > 1:174115/1‑86 (MQ=255) ctctCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAactac < 2:191060/90‑1 (MQ=255) ctcCGGCGTATATCGGCGGGGCGCGCTCCCCCATGCCCGACGCCCTTTTTAAAATTTGG‑CTGCGGGCCGGGGCCTGTTTTTATaatactt > 2:344675/1‑89 (MQ=255) cGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGAcc < 2:175132/90‑1 (MQ=255) gTATATCGGCGGGGGGCTCTCCCCCTTTCGCGGCGCCTTTTTTTAAATTTGGCC‑GCGCGCGGGGACCCGTTTTTTAAATACTGACCCTaa > 2:496208/1‑90 (MQ=255) atCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTaacaac < 2:388096/90‑1 (MQ=255) cggcggGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACgg < 2:174115/90‑1 (MQ=255) gTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCaa < 1:97074/90‑1 (MQ=255) | GCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAA > NZ_CP009273/3012836‑3013010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |