| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,465,390 | 0 | T | G | 22.2% | 28.8 / 11.1 | 18 | K90T (AAG→ACG) | BW25113_RS12285 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/7); new base G (2/2); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGC > NZ_CP009273/2465308‑2465470 | tggcggagtggctgaaggccacggaaccgggacaccaacaggtaatgcagagccttctcccaaaccaaGGTTTTCGAGAGCCGTTTGCAc < 1:215075‑M2/22‑1 (MQ=255) cggagtggctgaaggccacggaaccgggacaccaacaggtaatgcagagccttctccccaaaccaaGGTTTTCGAGAGCCGTTTGcacag > 2:266508‑M2/67‑88 (MQ=255) cAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCa > 1:190307/1‑90 (MQ=255) gTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAAtt < 2:115670/90‑1 (MQ=255) tGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCAc > 1:79377/1‑71 (MQ=255) tGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCAc < 2:79377/71‑1 (MQ=255) ccccTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCaa > 1:224206/1‑90 (MQ=255) ccccTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCaa > 1:117794/1‑90 (MQ=255) tGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGGGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACgg > 1:152965/1‑90 (MQ=255) tACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCtt > 2:180777/1‑90 (MQ=255) cATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGc < 1:56364/90‑1 (MQ=255) atgcagagccttctcccaaaccaaGGTTTTCGAGAGCCgtt > 1:232982‑M2/25‑41 (MQ=255) atgcagagccttctcccaaaccaaGGTTTTCGAGAGCCgtt < 2:232982‑M2/17‑1 (MQ=255) agagccttctcccaaaccaaGGTTTTCGAGAGCCGTTTGCAc < 1:63840‑M2/22‑1 (MQ=255) agagccttctcccaaaccaaGGTTTTCGAGAGCCGTTTGCAc > 2:63840‑M2/21‑42 (MQ=255) gCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACagcagc < 2:117794/90‑1 (MQ=255) aaccaaGGTTTTCGAGAGCCTTTTGCACCGTGCCGTcc > 2:144335‑M2/7‑38 (MQ=255) aaccaaGGTTTTCGAGAGCCTTTTGCACCGTGCCGTcc < 1:144335‑M2/32‑1 (MQ=255) tCGAGAGCGTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGTTTAACAGCAGCGCACGAAGCGCGGTAagcagc < 1:180777/90‑1 (MQ=255) tCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAagcagc < 2:110945/90‑1 (MQ=255) | ACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGC > NZ_CP009273/2465308‑2465470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |