| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,394,818 | 0 | T | C | 53.3% | 6.1 / 16.3 | 15 | D625G (GAT→GGT) | csrD | RNase E specificity factor CsrD |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base C (0/8); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTG > NZ_CP009273/3394738‑3394904 | aTTAAACGGCAGGTAAAACCGAGTATCTTTGTAAATTTTTTTCCCCGTTAGTATCAAGTGGTTGTGAGGACGCAAAAAAACCCCCTTGcc < 2:284528/90‑1 (MQ=255) tAAACGGCAGGTTAACCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCg < 1:31384/90‑1 (MQ=255) tAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTCCCCGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCg < 1:272602/90‑1 (MQ=255) tAAACGGCAGGTAAAACCGAGTATCTTTGTGAATTTTTTTTCCCGTTAGTATAAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCg < 2:200446/90‑1 (MQ=255) gCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTa > 1:44925/1‑90 (MQ=255) gTTAAACCGAGTATCTTTGTAAATTTTTTTTCCCGTTAGTATAAAGTGGCTGGGGGGACGCAAAAAAACCCCCTTGCCCGCCTGTAacac < 2:148380/90‑1 (MQ=255) aCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATAAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCgcgc < 2:60638/90‑1 (MQ=255) tttGTGAATTTTTTTTCACGTTAGTATCAAGTGGCTGGGAGGACGCAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAggg < 2:232094/90‑1 (MQ=255) gAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGc > 2:67515/1‑90 (MQ=255) ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTc < 2:44925/90‑1 (MQ=255) gggCTGTGGGGCCGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGt < 1:212593/89‑1 (MQ=255) ggCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTg > 1:166865/1‑90 (MQ=255) gAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTaa > 1:81985/1‑90 (MQ=255) gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTgg > 1:20484/1‑90 (MQ=255) aaaCCCCCTGCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTg < 2:166865/90‑1 (MQ=255) | ATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTG > NZ_CP009273/3394738‑3394904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |