| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,744,897 | 0 | A | C | 53.8% | 8.2 / 16.7 | 13 | V111G (GTT→GGT) | yiaT | MipA/OmpV family protein |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAA‑CGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCA > NZ_CP009273/3744819‑3744981 | cacccGTTGCTGTTACCCAGACCGCCCGCAGCTGCAGGGGCTTTCCCGGTCCCCCAA‑CGCTCGTGGTGATACCAGGCACCCCCCGCCATa < 2:30512/87‑1 (MQ=255) gTTGCTTTTACCCAGACCGCCCGCAGCTGCAGGGGTTTTCCCGGTCCCCCAA‑CGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGt < 2:58589/90‑1 (MQ=255) tATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAA‑CGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCAc > 2:280700/1‑90 (MQ=255) tGCAGAGGTTTTCACGGTCCCCCAA‑CGCTGGTGGTGATCCCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCAt < 1:280700/90‑1 (MQ=255) ggCTTTC‑CCGTTCCCCAACCGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATa < 2:72318/90‑1 (MQ=255) ttCACGGTTCCCCAA‑CGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGATGTTGCATGGCATAATCg > 1:220689/1‑90 (MQ=255) ccccAA‑CGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCg > 2:236217/1‑90 (MQ=255) ggtggtgATCCCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCaa < 2:57435/89‑1 (MQ=255) cGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCaa > 2:275808/1‑90 (MQ=255) ccAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCgg < 1:236217/90‑1 (MQ=255) aGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCa > 2:214173/1‑90 (MQ=255) gCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGt < 2:4588/58‑1 (MQ=255) gCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGt > 1:4588/1‑58 (MQ=255) | CAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAA‑CGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCA > NZ_CP009273/3744819‑3744981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |