Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927369,9000AC58.3% 7.1 / 10.4 12V370G (GTG→GGG) thiPthiamine/thiamine pyrophosphate ABC transporter permease ThiP
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/7);  total (4/8)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCA  >  NZ_CP009273/69831‑69976
                                                                     |                                                                            
aGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTc                                                          >  1:30798/1‑90 (MQ=255)
           gCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCg                                               >  1:269247/1‑90 (MQ=255)
            ccGGTACCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                              <  1:41809/90‑1 (MQ=255)
                              cccGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCAt                            <  1:59401/90‑1 (MQ=255)
                                cGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcgcg                                      <  1:138504/78‑1 (MQ=255)
                                cGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcgcg                                      >  2:138504/1‑78 (MQ=255)
                                 ggCAGGGGGGGGACCAACATGCCCCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc                         <  2:269247/90‑1 (MQ=255)
                                        gggggATCAACATGCCCCTCCTCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcatca                  <  1:144758/87‑1 (MQ=255)
                                                       cGCTCATCTCCAGCCCCTCCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc   <  2:194879/90‑1 (MQ=255)
                                                       cGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc   <  1:3764/90‑1 (MQ=255)
                                                       cGCTCACCCCCACCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc   <  2:30798/90‑1 (MQ=255)
                                                        gCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGca  >  1:109431/1‑90 (MQ=255)
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AGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCA  >  NZ_CP009273/69831‑69976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑