| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 765,670 | 0 | G | T | 54.5% | 6.7 / 13.6 | 11 | S746I (AGT→ATT) | mngB | mannosylglycerate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base T (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATGGG > NZ_CP009273/765591‑765759 | gggCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGt > 2:38946/1‑90 (MQ=255) cacaACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAg > 1:123456/1‑90 (MQ=255) catGGTCTGTTTTTTTGTCGCTTAATTTTATTGATTTATCCGGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:237262/88‑1 (MQ=255) cGTGGTTTTTTTTTTTGTCGCCTAATTTTATTGATTTATCCCGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 2:76414/90‑1 (MQ=255) cGTGGTCTGCTTTTTTGTCGCCTAATTTTATTGATTTATCCGGGTCCGCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 2:148783/90‑1 (MQ=255) cGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:38946/90‑1 (MQ=255) cGTGGTCTGCTTTCTTGTCCCCTAATTTTATTGATTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:194707/90‑1 (MQ=255) gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTc > 1:55942/1‑90 (MQ=255) cTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCa > 1:57257/1‑90 (MQ=255) tttttGTCGCCTAAGTTTATTGATTTATCCCGGTCCCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACa < 2:298251/87‑1 (MQ=255) cGCTTAATTTTTTTGATTTATCCCGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTac < 2:57257/90‑1 (MQ=255) gTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:226452/56‑1 (MQ=255) gTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg > 2:226452/1‑56 (MQ=255) gTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATggg > 2:150306/1‑90 (MQ=255) | GGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATGGG > NZ_CP009273/765591‑765759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |