Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,178,033	C→T	A919V (GCG→GTG)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,178,033	0	C	T	100.0%	30.0 / NA	9	A919V (GCG→GTG)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

ACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGA  >  NZ_CP009273/4177949‑4178117                                                                                     |                                                                                     aCTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGTGGcac                                                                                 <  2:11253/90‑1 (MQ=255)                              cGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTg                                                    >  2:236394/1‑90 (MQ=255)                                 gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGAcc                                                 <  1:276403/90‑1 (MQ=255)                                         gcgTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTAc                                         <  1:39577/90‑1 (MQ=255)                                           gTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGt                                       <  1:31280/90‑1 (MQ=255)                                           gTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGt                                       <  2:98419/90‑1 (MQ=255)                                                            aaGGGTGAAGCAATCGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCg                      >  1:8356/1‑90 (MQ=255)                                                                          cGGTGTTATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgc        <  1:236394/90‑1 (MQ=255)                                                                                tATCGTGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGa  >  2:47590/1‑90 (MQ=255)                                                                                     |                                                                                     ACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGA  >  NZ_CP009273/4177949‑4178117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑