Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273349,9990TG48.7% 129.1 / 63.8 113V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (6/52);  new base G (55/0);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 1.11e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC  >  NZ_CP009273/349911‑350084
                                                                                         |                                                                                      
aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTg                                                                                       <  2:514874/90‑1 (MQ=255)
  cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGaa                                                                                     >  1:46632/1‑90 (MQ=255)
    ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAg                                                                                   >  1:411629/1‑90 (MQ=255)
    ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGC‑GGGGGGTGGGGGGGAAg                                                                                   >  2:1479558/1‑90 (MQ=255)
     tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGa                                                                                  <  1:14998/90‑1 (MQ=255)
     tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGa                                                                                  <  2:405665/90‑1 (MQ=255)
     tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGa                                                                                  >  1:983730/1‑90 (MQ=255)
     tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGa                                                                                  >  1:141231/1‑90 (MQ=255)
      gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGAt                                                                                 <  2:550497/90‑1 (MQ=255)
      gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAt                                                                                 >  1:482298/1‑90 (MQ=255)
        cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgc                                                                               <  1:363700/90‑1 (MQ=255)
        cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgc                                                                               <  1:657858/90‑1 (MQ=255)
         gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAGGTGGC‑GGTGGTTGGGGTGAAAGATgcg                                                                              <  2:1292113/90‑1 (MQ=255)
          ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgcgc                                                                             <  1:384600/90‑1 (MQ=255)
          ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgcgc                                                                             <  1:185588/90‑1 (MQ=255)
           gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGAAAGATGCGCt                                                                            >  1:568581/1‑90 (MQ=255)
              tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGaa                                                                         <  1:1323747/90‑1 (MQ=255)
              tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTgga                                                                         >  1:1030301/1‑88 (MQ=255)
               cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGaaa                                                                        <  2:827648/90‑1 (MQ=255)
                          gcgcgAGATTGAAGGGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAggtggg                                                             >  1:1100302/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGGAAA‑ATGGGCTGAAAGGGGAgggggg                                                             >  2:288677/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAAGGGAgggggg                                                             >  1:163786/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAgggggg                                                             >  1:129457/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAggtggc                                                             >  1:305482/1‑90 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAggtggc                                                             >  1:1437048/1‑90 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCGGGAAGGGGAgggggg                                                             >  1:243372/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAAATGGGCTGGAAGGGCAgggggg                                                             >  1:767486/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGAAAGATGCGCTGAAAGGGGAgggggg                                                             >  1:1465381/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGTGAAAGATGCGCTGAAAGGGCAggtggg                                                             >  1:1033227/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAAGGGCTGGAAGGGGAgggggg                                                             >  2:485579/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAAAGCGCTGAAAGGGCAgggggg                                                             >  2:896430/1‑89 (MQ=255)
                          gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGACGAAGTGGC‑GGTGGGTTGGGGGAAAGATGCGCTGGAAGGGGAgggggg                                                             >  1:455062/1‑89 (MQ=255)
                            gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑gg                                                          <  1:1143570/90‑1 (MQ=255)
                                       gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTc                                               <  2:1202667/90‑1 (MQ=255)
                                       gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTc                                               <  2:912185/90‑1 (MQ=255)
                                       gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTc                                               <  1:288677/90‑1 (MQ=255)
                                       gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTc                                               <  1:485579/90‑1 (MQ=255)
                                       gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTc                                               <  2:366394/90‑1 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAAATGCGCTGAAAAGGCAGGGGGG‑GGGGGGGGTTGTCAt                                             >  1:1206336/1‑90 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGAAAGATGCGCTGGAAGGGGAGGTGGG‑GGTGGCGGTTGTTAt                                             >  1:951374/1‑90 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGTTAAAAAGCGCTGAAAGGGCAGGGGGC‑GGGGGGGTTTGTCAt                                             >  1:1048614/1‑90 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGCGGTTGTCAt                                             >  1:547480/1‑90 (MQ=255)
                                         gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGAAAGAGGCGCGGAAAAGGGAGGGGGCGGGGGGGGTTTGTct                                              >  1:1171841/1‑89 (MQ=255)
                                           gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTc                                           <  1:64796/90‑1 (MQ=255)
                                           gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGGGGGGGGG‑GGGGGCGTTTGTCGTTc                                           >  2:1261095/1‑90 (MQ=255)
                                           gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGAAAGAAGCGCCGAAAGGGCGGGGGGG‑GGGGGGGGTTGTGATTc                                           >  2:516031/1‑90 (MQ=255)
                                              tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCCGCAAGGGCAGGTGGC‑GGGGGCGGTTGTCATTTCGa                                        >  1:874135/1‑90 (MQ=255)
                                              tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAAGCGCTGAAAGGGGAGGGGGC‑GGGGGGGGTTGTCATTCCGa                                        >  1:1378698/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGTGCTGAAAAGGGAGGGGGG‑GGGGGCGTTTTTCATTTCGTAagag                                   >  2:1534579/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGGGCCGAAAGGGCAGGGGGC‑GGTGGTGTTTTTCTTTTCCAAaaag                                   >  1:1514584/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGG‑GGGGGCGTTTGTCATTCCGCAagag                                   >  1:1079918/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGGTTGTCATTCCGGAagag                                   >  1:86246/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGG‑GGGGGCGGTTGTCATTCCGAAaaag                                   >  1:3917/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGCGGTTGTCATTCCGAAagag                                   >  1:621808/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGGGCCGGAAAGGGAGGGGGG‑GGGGGGGGTTGTTCTTTCGAAagag                                   >  2:1577225/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGC‑GGGGGGGGTTTTCATTCCCAAaaag                                   >  1:906445/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTTGGGGGAAAAATGCGCTGCAAGGGGAGGAGGC‑GGGGGCGGTTTTCATTTCTGAagag                                   >  1:1224366/1‑90 (MQ=255)
                                                   aGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGG‑GGGGGGGGTTGGTATTTCGAAagaa                                   >  2:1147633/1‑89 (MQ=255)
                                                       aTCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGAAAGAGAGCg                               >  2:371799/1‑90 (MQ=255)
                                                       aTCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAGGCGCTGGAAGGGGAGGGGGG‑GGGGGGGGTTGTTCTTTCGGAagagaggg                               >  2:1067663/1‑88 (MQ=255)
                                                       aTCCGGGCGTTGCCGAAGTGGC‑GGATGTTGGGGGGAAAAAGGCGCTGAAAAGGCCGGGGGG‑GGGGGGGGTTGTCCTTTCGAAagagaggg                               >  2:976575/1‑88 (MQ=255)
                                                        tCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGGAAGATGGGCTGAAAGGGGGGGGGGG‑GGGGGGGGTTGTCATTTCGAAAAAGAGGGa                              >  2:1528549/1‑90 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:899795/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  1:1528549/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  1:1444507/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:522334/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:893865/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:305482/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  1:62835/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  1:735969/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:1514584/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:1269799/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:1117896/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  1:896430/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:1279227/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:1402090/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:1437048/90‑1 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGG‑GGGGGGGGTTTTCCTTCCGGAAAAGAGCggc                             >  2:1376313/1‑88 (MQ=255)
                                                         ccGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAGGCGCTGAAAGGGGCGGGGGG‑GGTGGGGTTTGTCGTTCCGGAAGAGAGCGAc                             >  2:1553425/1‑90 (MQ=255)
                                                           gggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg                           <  1:560418/90‑1 (MQ=255)
                                                           gggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg                           <  2:55794/90‑1 (MQ=255)
                                                           gggCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGAAAAGGGAGGGGGG‑GGGGGGGGTTTTTATTTCCAAAAAGAGCGACAg                           >  2:493613/1‑90 (MQ=255)
                                                                ttGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                      <  2:1086795/90‑1 (MQ=255)
                                                                ttGCCGAAGTGGC‑GGGGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                      <  1:1213808/90‑1 (MQ=255)
                                                                 tGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGaa                                                                         <  1:1100471/39‑1 (MQ=255)
                                                                 tGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGaa                                                                         >  2:1100471/1‑39 (MQ=255)
                                                                 tGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGa                     >  1:160511/1‑90 (MQ=255)
                                                                 tGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGG‑GGGGGCGTTTGTCATTCCCAAAGAGAGCGACAGGCTGGa                     >  1:1003804/1‑90 (MQ=255)
                                                                  gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                    <  2:888069/90‑1 (MQ=255)
                                                                  gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                    <  2:1246993/90‑1 (MQ=255)
                                                                     gAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                 <  1:867265/90‑1 (MQ=255)
                                                                      aaGTGGC‑GGTGGGTGGGGGGAAAAATGCGCTGAAAGGGGAGGGGGG‑GGGGGCGGTTGTCATTCCCAAAGAGAGCGACAGGCTGGGAGATc                >  2:411566/1‑90 (MQ=255)
                                                                          ggC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa            <  1:1261095/90‑1 (MQ=255)
                                                                          ggC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAAAGAGGGACAGTCTGGGAGATCGTgg            >  2:409146/1‑89 (MQ=255)
                                                                            c‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCgtggtg          >  2:409876/1‑90 (MQ=255)
                                                                                   ttGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc    >  2:1260347/1‑90 (MQ=255)
                                                                                    tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa   <  2:746333/90‑1 (MQ=255)
                                                                                    tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa   <  1:1100935/90‑1 (MQ=255)
                                                                                     ggggTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt              <  1:250539/78‑1 (MQ=255)
                                                                                     ggggTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc  <  1:577398/90‑1 (MQ=255)
                                                                                         |                                                                                      
AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC  >  NZ_CP009273/349911‑350084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑