| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 799,325 | 0 | G | T | 66.7% | ‑3.7 / 18.2 | 18 | V123L (GTG→TTG) | ybhJ | hydratase |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base T (12/0); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA > NZ_CP009273/799237‑799412 | tAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTtgt < 1:796055/90‑1 (MQ=255)tAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTtgt < 1:1487995/90‑1 (MQ=255) actcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGTTTTTTTGGCTcc > 2:567723/1‑90 (MQ=255) tCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCATAttttgg > 1:1042770/1‑86 (MQ=255) ggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCTGTcatc < 1:1466262/90‑1 (MQ=255) cACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatcca < 2:995069/90‑1 (MQ=255) cTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatccatc < 1:1546882/90‑1 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTGTTTTTTTGTCTCCCCCTATTTGGGGCATTCACCAAAATATggg > 2:1528023/1‑88 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTGTTTTTTTGCCCCCGCATATTTGGGTTCTTCCTCAatatatttg > 1:870288/1‑87 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCATATTGCGGGCATCCATCAATATATGCg > 1:122034/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGTTTTTTTTTCCCCCCCTATTTCGGGCCTCCCTCAatataatcg > 2:1515532/1‑86 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCGCATATTTCGGGCATCCCTCAATATATTCg > 1:1149567/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCGCATATTGCGGTCATCCATCAATATATGCg > 1:196711/1‑90 (MQ=255) tGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGTTTTTTTGTCCCCCCCTATTTGGGGCCTCCCCCAATATATGGGTGGgatggt > 2:722185/1‑88 (MQ=255) tATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCCTATTTCGGTCATCCATCAATATATTCGGGAGATGATGGGAggggggg > 1:1549272/1‑88 (MQ=255) ggcCCAGCGTTATGGCGGGATTTTTTTGCCCCCCCATATTGCGGGCCTCCATCAATATATGGGGGAGGTGATGGCAGGGGGCGGGaaaaa > 1:1260611/1‑89 (MQ=255) gggaTTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATTCGTGAGGTGATGGCAGGGGGCGGCCAAATGATCCTCGGGAAAGAc > 2:518328/4‑90 (MQ=255) ggTATTTTTTTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTg > 1:1452178/1‑49 (MQ=255) ggTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTg < 2:1452178/49‑1 (MQ=255) ttGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCcaca < 1:1528023/90‑1 (MQ=255) tgtgCCTCCACATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCca < 1:890191/87‑1 (MQ=255) tgtgCCTCCACATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCca > 2:890191/1‑87 (MQ=255) | TAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA > NZ_CP009273/799237‑799412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |